Phospho-GRIN2B-Y1474 Rabbit pAb for WB, ELISA - Q13224

Core Product Specifications and Parameters

Biological Background: GRIN2B Function and Localization

• Component of NMDA receptors, functioning as ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg2+ (PubMed:24272827, PubMed:24863970, PubMed:26875626).
  Related references: PMID:24272827, PMID:24863970, PMID:26875626
• Channel activation requires L-glutamate binding to GluN2, glycine or D-serine binding to GluN1, plus membrane depolarization to relieve Mg2+ block (PubMed:24272827, PubMed:24863970, PubMed:26875626).
  Related references: PMID:24272827, PMID:24863970, PMID:26875626
• The GluN2B subunit confers distinct activation, deactivation, and desensitization kinetics, pH sensitivity, Ca2+ permeability, and allosteric modulation (PubMed:26875626, PubMed:28095420, PubMed:28126851).
  Related references: PMID:26875626, PMID:28095420, PMID:28126851
• Participates in synaptic plasticity underlying learning and memory, contributing to long-term depression (LTD) in hippocampus.
• Phosphorylation at Ser-1303 by DAPK1 at extrasynaptic sites enhances channel activity, leading to injurious Ca2+ influx and neuronal death in stroke.
• Primarily expressed in the fronto-parieto-temporal cortex and hippocampal pyramidal cells, with lower expression in the basal ganglia.
• Localizes to the cell membrane, postsynaptic membrane, dendrites, late endosomes, lysosomes, cytoplasm, and cytoskeleton.
• Key features: glycoprotein, disulfide bonds, phosphorylation, and metal-binding.

Experimental Guidance and Technical Tips

• This antibody is raised against a phosphorylated peptide at Tyr1474; ensure samples are prepared with phosphatase inhibitors to preserve the phospho-epitope.
• The predicted molecular weight of GRIN2B is approximately 166 kDa; use an appropriate gel percentage (e.g., 6–8% SDS-PAGE) and extended transfer times for efficient western blot detection.
• Cross-reactivity with human, mouse, and rat is indicated; validate in relevant brain lysates (e.g., cortex, hippocampus) for your experimental system.
• For ELISA, consider coating the plate with phosphorylated peptide to confirm specificity, and include non-phosphorylated controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips diagnostic manufacturers and research teams with a comprehensive portfolio of IVD raw materials, including validated antibody pairs and bulk ancillary reagents. This phospho-GRIN2B (Y1474) antibody supports neuroscience-focused immunoassay development, and our sourcing team can assist with rare targets and custom formulations to accelerate your project from concept to clinic.