Phospho-GluR2/GRIA2-S880 Rabbit pAb for WB - P42262

Core Product Specifications and Parameters

Biological Background: Glutamate receptor 2 Function and Localization

• Glutamate receptor 2 (GluR2), also known as GluR-B, GluR-K2, and AMPA-selective glutamate receptor 2, is encoded by the GRIA2 gene. It belongs to the ionotropic glutamate receptor family.
• Functions as a ligand-gated cation channel, activated by L-glutamate and agonists such as AMPA, quisqualic acid, and kainic acid, mediating fast excitatory synaptic transmission in the central nervous system (PubMed:14687553). Related references: PMID:14687553
• Upon agonist binding, the receptor undergoes conformational change, opening the channel for monovalent and divalent cations like sodium and calcium (PubMed:20614889, PubMed:8003671). Related references: PMID:20614889, PMID:8003671
• The receptor rapidly desensitizes, but in the presence of auxiliary subunits (CACNG4, CACNG7, CACNG8), it exhibits resensitization, characterized by delayed current accumulation upon continued agonist application.
• Subcellularly localized to the cell membrane, specifically the postsynaptic density membrane, where it participates in synaptic plasticity and signal transduction.
• Post-translational modifications include phosphorylation (e.g., at Ser880), palmitoylation, and glycosylation, which regulate receptor trafficking and function. The protein also undergoes RNA editing and alternative splicing.
• Associated keywords: 3D-structure, Alternative splicing, Autism spectrum disorder, Disease variant, Epilepsy, Intellectual disability, Ligand-gated ion channel, Phosphoprotein, Synapse.

Experimental Guidance and Technical Tips

• This antibody was raised against a phospho-specific peptide corresponding to residues surrounding S880 of human GluR2/GRIA2; confirm specificity by treating samples with lambda phosphatase or blocking with non-phosphorylated peptide as a negative control.
• For Western blotting, a band at approximately 99 kDa is expected; consider using fresh protease and phosphatase inhibitors during sample preparation to preserve phosphorylation.
• Validate the antibody in relevant human, mouse, or rat tissue or cell lysates expressing GluR2, such as brain tissue, to confirm specific detection.
• Given the role of S880 phosphorylation in AMPA receptor endocytosis and synaptic plasticity, this antibody may be useful for studying neurological disease models; appropriate experimental conditions should be optimized by the end user.

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CamelBio provides diagnostic manufacturers and research institutions with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For neuroscience and synaptic research, we offer rare-target raw-material sourcing and customized support for glutamate receptor-related biomarkers. Our one-stop platform bridges the gap from concept to clinic, ensuring reliable supply for your assay development and production needs.