Phospho-GluR1/GRIA1 (S836) Rabbit Polyclonal Antibody for WB - P42261

Core Product Specifications and Parameters

Biological Background: GluR1/GRIA1 Function and Localization

• Glutamate receptor 1 (GluR1/GRIA1), also known as AMPA-selective glutamate receptor 1, is a key ionotropic receptor mediating fast excitatory synaptic transmission in the central nervous system. Related references: PMID:1311100 PMID:20805473 PMID:21172611
• As a ligand-gated cation channel, GluR1 opens upon L-glutamate binding, allowing influx of sodium and calcium ions, and rapidly desensitizes to regulate synaptic responses.
• The receptor can undergo resensitization when associated with auxiliary subunits (CACNG2/4/7/8), leading to delayed current accumulation (PubMed:21172611). Related references: PMID:21172611
• Calcium permeability varies with subunit composition; homomeric GluR1 channels are calcium-permeable, while heteromeric channels containing edited GRIA2 are calcium-impermeable.
• Subcellular localization includes the postsynaptic density, dendritic spines, cell membrane, and intracellular compartments such as the endoplasmic reticulum and endosomes, reflecting its dynamic trafficking in synaptic plasticity.
• Widely expressed in the brain, GluR1 is critical for learning, memory, and neuropathology.
• Phosphorylation at multiple sites, including Ser836, modulates receptor function and synaptic strength; the protein is classified as a phosphoprotein.
• Alternative splicing and disease-associated mutations link GRIA1 to intellectual disability and other neurological disorders.

Experimental Guidance and Technical Tips

• This antibody is generated against a phospho-specific peptide, ensuring selectivity for GluR1 phosphorylated at Ser836. Verify phosphorylation-dependent signal by treating samples with lambda phosphatase or by blocking with the phospho-peptide.
• For Western blot, load brain lysates from human, mouse, or rat as positive controls; the predicted band is ~102 kDa. Use appropriate stimulation protocols (e.g., chemical LTP) to enhance phosphorylation.
• Optimize blocking (5% BSA or non-fat milk) and washing conditions to reduce non-specific binding; consider using TBS-T as the buffer.
• Cross-reactivity across human, mouse, and rat makes this antibody suitable for comparative neuroscience studies; however, always validate the antibody in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For neuroscience and synaptic plasticity research, we offer validated antibody pairs, optimized polyclonal antibodies like this anti-phospho-GluR1 (Ser836), and bulk ancillary reagents to support your assay development. Partner with CamelBio for reliable, scalable supply of critical raw materials, from rare target sourcing to large-volume production.