Phospho-GATA1-S310 Rabbit Polyclonal Antibody for WB, ELISA - P15976

Core Product Specifications and Parameters

Biological Background: GATA1 Function and Localization

• GATA1 functions as a transcriptional activator or repressor and is a master regulator of erythroid development. Related references: PMID:35030251
• It binds to the consensus DNA sequence 5'-[AT]GATA[AG]-3' in regulatory regions of erythroid-specific genes.
• It activates transcription of key erythroid genes, including HBB (β-globin), HBG1/2 (γ-globins), ALAS2 and HMBS. Related references: PMID:24245781
• Subcellular localization is nuclear, consistent with its role as a transcription factor.
• Primarily expressed in erythrocytes.
• GATA1 undergoes multiple post-translational modifications including phosphorylation, acetylation, and sumoylation, and contains two zinc‑finger domains essential for DNA binding.
• Mutations in GATA1 are associated with hereditary hemolytic anemias and X-linked thrombocytopenia.

Experimental Guidance and Technical Tips

• Due to the phospho-specific immunogen, this antibody is expected to primarily detect GATA1 phosphorylated at Ser310. Include phosphatase inhibitors in lysis buffers for Western blot to preserve phosphorylation.
• Consider treating samples with phosphatase or performing phospho‑peptide competition assays to confirm signal specificity.
• For ELISA, optimize coating conditions and antibody dilutions empirically; cross-reactivity with human, mouse, and rat GATA1 enables multi‑species studies.
• Validation of antibody performance in the relevant sample system and application is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated phospho-specific antibodies, bulk ancillary reagents, and rare-target sourcing services. This Phospho-GATA1-S310 Rabbit pAb is part of our expanding portfolio for hematology and transcription factor research, supporting biomarker discovery and assay development.