Phospho-GAB1 (Tyr659) Rabbit Polyclonal Antibody for WB, ELISA - Q13480

Core Product Specifications and Parameters

Biological Background: GRB2-associated-binding protein 1 Function and Localization

• GAB1 (GRB2-associated-binding protein 1) is a scaffolding adapter protein that mediates signaling downstream of multiple receptor tyrosine kinases.
• It participates in intracellular signaling cascades triggered by activated receptors such as FGFR1, EGFR, and INSR.
• Specifically involved in MET/HGF-signaling pathway. Related references: PMID:29408807
• A member of the GAB family of adapters, sharing structural motifs like a pleckstrin homology domain and multiple tyrosine phosphorylation sites that recruit SH2 domain-containing proteins.
• Heavily phosphorylated on tyrosine residues (including Y659) upon receptor activation, which is critical for its interaction with downstream effectors.
• Mutations in GAB1 are linked to non-syndromic deafness (DFNB26), highlighting its role in auditory function.
• Undergoes acetylation and alternative splicing, which may modulate its activity.
• 3D structure information is available, providing insights into its molecular architecture.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide corresponding to residues surrounding Tyr659 of human GAB1. Ensure that your detection system is specific for the phosphorylated form; consider using a non-phospho-specific antibody as a control.
• For Western blot, predicted molecular weight is approximately 77 kDa. Use appropriate gel percentage and transfer conditions for proteins in this mass range. Validate band specificity using phosphatase-treated lysates or relevant inhibitors.
• For ELISA, the antibody can be used as a capture or detection reagent; optimization of coating conditions and antibody concentration is recommended. Include phosphorylated peptide as a positive control.
• Cross-reactivity has been demonstrated with human and mouse GAB1. When using mouse samples, be aware of potential background from endogenous mouse immunoglobulins if using anti-rabbit secondary antibodies.
• As GAB1 is involved in multiple signaling pathways (EGFR, INSR, MET/HGF), consider appropriate cell stimulation with growth factors (e.g., EGF, insulin, HGF) before sample preparation to induce phosphorylation at Tyr659.
• Since the antibody is a rabbit polyclonal, lot-to-lot consistency should be verified for critical quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, connecting diagnostic manufacturers and research institutions with high-quality antibody reagents and ancillary products. This anti-phospho-GAB1 (Tyr659) antibody supports signaling pathway research and assay development for EGFR, MET, and insulin receptor pathways. We also offer customized sourcing of rare targets, validated antibody pairs, and optimized bulk polyclonal antibodies to accelerate your project from concept to clinic.