Phospho-FOXO3A-S425 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O43524

Core Product Specifications and Parameters

Biological Background: Forkhead box protein O3 Function and Localization

• Forkhead box protein O3 (FOXO3A), also known as AF6q21 protein or Forkhead in rhabdomyosarcoma-like 1 (FKHRL1), is a ubiquitously expressed transcription factor encoded by the FOXO3 gene (human gene ID 2309).
• FOXO3A acts as a transcriptional activator that recognizes the DNA consensus 5'-[AG]TAAA[TC]A-3', regulating key processes like apoptosis and autophagy. Related references: PMID:10102273, PMID:16751106, PMID:21329882
• In response to nutrient starvation, FOXO3A enters the nucleus upon dephosphorylation and upregulates autophagy genes (e.g., GABARAP1L, MAP1LC3B, ATG12), promoting skeletal muscle protein degradation.
• FOXO3A triggers apoptosis in the absence of survival factors, notably causing neuronal cell death under oxidative stress. Related references: PMID:10102273, PMID:16751106
• Following phosphorylation by MAPKAPK5, FOXO3A induces miR-34b and miR-34c, which post-transcriptionally suppress MYC oncogene expression. Related references: PMID:21329882
• FOXO3A shuttles between the cytoplasm and nucleus, and under metabolic stress it translocates to the mitochondrial matrix and outer membrane to enhance mtDNA transcription. Related references: PMID:23283301
• Key post-translational modifications include phosphorylation (e.g., at Ser425), acetylation, methylation, and ubiquitination, which govern its activity, localization, and stability.
• FOXO3A is involved in regulatory T-cell differentiation by activating FOXP3 expression, and in chondrogenic commitment of skeletal progenitors under low lipid conditions. Related references: PMID:30513302

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide surrounding serine 425; to preserve this labile modification, add phosphatase inhibitors during sample preparation.
• For Western blot (WB), consider loading a positive control such as lysate from cells treated with an inducer of FOXO3A phosphorylation (e.g., oxidative stress or serum starvation) and validate band specificity with a phosphatase‑treated sample.
• Immunohistochemistry (IHC‑P) may require antigen retrieval optimization; include a blocking step with phospho‑specific considerations to reduce non‑specific binding.
• ELISA protocols should use the recommended coating conditions and include a standard curve; cross‑reactivity across human, mouse, and rat suggests the epitope is conserved, but always confirm performance in your matrix of interest.
• Protein weight is approximately 71 kDa; verify detection at the expected size and account for possible shift due to post‑translational modifications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with streamlined access to premium IVD raw materials. This phospho-FOXO3A-S425 antibody exemplifies our portfolio of validated phospho-specific antibodies designed for apoptosis and autophagy research. Beyond individual reagents, we supply optimized antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets—accelerating your concept‑to‑clinic journey.