Phospho-FOXO3A-S253 Rabbit pAb for WB, IHC-P, ELISA - O43524

Core Product Specifications and Parameters

Biological Background: FOXO3A Function and Localization

• FOXO3A is a transcriptional activator that recognizes the DNA sequence 5'-[AG]TAAA[TC]A-3' and regulates critical processes including apoptosis and autophagy.
  Related references: PMID:10102273, PMID:16751106, PMID:21329882
• In skeletal muscle, FOXO3A acts as a positive regulator of autophagy by entering the nucleus upon dephosphorylation and activating autophagy genes (GABARAP1L, MAP1LC3B, ATG12), leading to protein breakdown.
• The protein triggers apoptosis in the absence of survival factors, including neuronal cell death under oxidative stress.
  Related references: PMID:10102273, PMID:16751106
• FOXO3A participates in post-transcriptional regulation of MYC by promoting expression of miR-34b and miR-34c, which bind the MYC 3'UTR and inhibit translation.
  Related references: PMID:21329882
• Upon metabolic stress, FOXO3A translocates to mitochondria where it enhances mtDNA transcription.
  Related references: PMID:23283301
• FOXO3A is a key regulator of regulatory T-cell (Treg) differentiation through activation of FOXP3 expression.
  Related references: PMID:30513302
• Subcellular localization includes the cytoplasm, cytosol, nucleus, mitochondrial matrix, and mitochondrial outer membrane. Its activity is regulated by post-translational modifications such as phosphorylation, acetylation, and ubiquitination.
• FOXO3A is ubiquitously expressed across tissues.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Ser253; the antibody may preferentially recognize the phosphorylated form of FOXO3A. For Western blot, consider treating samples with phosphatase to confirm phosphorylation-specific detection.
• For immunohistochemistry (IHC-P), antigen retrieval conditions and tissue fixation methods should be optimized. Validate staining specificity with appropriate positive and negative tissue controls.
• The antibody cross-reacts with human, mouse, and rat FOXO3A, making it suitable for multispecies studies. Verify species-specific reactivity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop sourcing for IVD raw materials, including validated antibodies, bulk reagents, and rare target procurement. For FOXO3A-related research—spanning apoptosis, autophagy, and transcription regulation—we can supply the necessary components to accelerate your assay development and clinical translation.