Phospho-Filamin A-S2152 Rabbit pAb for WB, ELISA - P21333

Core Product Specifications and Parameters

Biological Background: Filamin-A Function and Localization

• Filamin-A (FLNA) is a major actin-binding protein that crosslinks actin filaments into orthogonal networks and tethers them to membrane glycoproteins, serving as a scaffold for a wide range of cytoplasmic signaling proteins.
• It localizes to the cell cortex, cytoskeleton, perikaryon, and specialized cell projections such as growth cones and podosomes, where it organizes the actin cytoskeleton.
• Filamin-A is essential for cell adhesion, cell-cell contacts, and adherens junction dynamics during the development of blood vessels, heart, and brain.
• It is required for SEMA3A-induced growth cone collapse during axon guidance, linking semaphorin signaling to actin remodeling. Related references: PMID:25358863
• Filamin-A is ubiquitously expressed and has a molecular weight of ~281 kDa; multiple isoforms arise from alternative splicing, though this product targets a phosphorylation site conserved in the canonical sequence.
• The protein is subject to multiple post-translational modifications including phosphorylation, acetylation, isopeptide bond formation, and ubiquitin conjugation; phosphorylation at S2152 is a dynamically regulated event.
• Mutations in FLNA are associated with a spectrum of human diseases, including periventricular nodular heterotopia, otopalatodigital syndromes, and cardiac valvular dysplasia.

Experimental Guidance and Technical Tips

• Because the immunogen is a synthetic phosphopeptide around S2152, include phosphatase inhibitors during sample preparation to preserve the phosphorylation state for Western blotting.
• Verify antibody specificity by competition with the non-phosphorylated peptide or by treating samples with lambda phosphatase; a loss of signal confirms phospho-specificity.
• The large size of Filamin-A (281 kDa) may require low-percentage SDS-PAGE gels (e.g., 6%) and extended transfer times; consider wet transfer at 30 V overnight.
• For ELISA, consider pairing this phospho-specific antibody with a total Filamin-A antibody to enable phospho/total ratio measurements.
• Cross-reactivity is reported for human, mouse, and rat; validate in your specific tissue or cell system if working with other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For research areas such as actin cytoskeleton dynamics, cell signaling, and Filamin A-associated diseases, we also offer rare target raw-material sourcing and technical consultation to support your project from concept to clinic. Contact us to explore how we can supply this product or customized alternatives for your development needs.