Phospho-FGFR3-Y724 Rabbit pAb - P22607

Core Product Specifications and Parameters

Biological Background: FGFR3 Function and Localization

• Encoded by the FGFR3 gene (also known as JTK4), fibroblast growth factor receptor 3 is a transmembrane tyrosine kinase that serves as a cell-surface receptor for fibroblast growth factors.
• Plays a critical role in the regulation of chondrocyte differentiation, proliferation, and apoptosis, and is essential for normal skeletal development and postnatal bone mineralization.
• Ligand binding triggers activation of multiple downstream signaling pathways, including PLCG1, leading to production of DAG and IP3, and FRS2-mediated recruitment of GRB2, GAB1, PIK3R1, and SOS1, resulting in RAS-MAPK and AKT1 pathway activation.
• Mutations causing constitutive kinase activation or impaired receptor maturation, internalization, and degradation lead to aberrant signaling and are associated with skeletal dysplasias and cancers; secreted isoform 3 may interfere with FGF signaling.
• Subcellular localization includes the cell membrane, cytoplasmic vesicles, endoplasmic reticulum, and a secreted form; expression is detected in brain, kidney, and testis, with high fetal expression in kidney, lung, small intestine, and brain.
• Isoform 2 is predominantly detected in epithelial cells, while isoform 1 and 2 are found in fibroblastic cells; the protein undergoes alternative splicing and N-linked glycosylation.
• Classified as a phosphoprotein and receptor tyrosine kinase, FGFR3 is implicated in diseases including craniosynostosis, deafness, dwarfism, and ectodermal dysplasia.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is approximately 88 kDa; consider using an appropriate gel percentage (e.g., 8–10% SDS-PAGE) and optimizing transfer conditions. Validate detection in your sample system as post-translational modifications and isoforms may cause mobility shifts.
• The immunogen is a synthetic phosphopeptide corresponding to Tyr724 of human FGFR3; to confirm phosphorylation specificity, include treatment with lambda phosphatase or use a non-phosphorylated peptide blocking control in ELISA.
• This antibody cross-reacts with human and mouse FGFR3; verify reactivity in other species before use.
• For ELISA, optimize coating antigen concentration and antibody dilution using the phosphorylated peptide as a positive control. Consider a standard indirect ELISA protocol.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized supplier of IVD raw materials, CamelBio provides a one-stop platform for sourcing validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. We support diagnostic and research communities with reliable access to critical reagents for tyrosine kinase signaling research, including this phospho-specific FGFR3 antibody. From early-stage assay development to clinical application, our team facilitates efficient procurement and customized sourcing for rare targets.