Phospho-FAK-Y576/577 Rabbit Polyclonal Antibody for WB, ELISA - Q05397

Core Product Specifications and Parameters

Biological Background: FAK Function and Localization

• Focal adhesion kinase 1 (FAK, PTK2) is a non-receptor protein-tyrosine kinase that regulates cell migration, adhesion, spreading, and actin cytoskeleton reorganization.
• FAK plays essential roles in embryonic development, angiogenesis, cardiomyocyte migration, and nervous system development.
• The full-length isoform 1 mediates signaling from integrins, growth factor receptors, and GPCRs, activating PI3K/AKT and MAPK pathways.
• Isoform 6 (FRNK) lacks the kinase domain and functions as a dominant-negative inhibitor of FAK phosphorylation and signaling.
• FAK is localized at focal adhesions, cell membrane, cytoplasm, centrosomes, and nucleus, indicating dynamic shuttling.
• Phosphorylation at Tyr576 and Tyr577 in the kinase domain activation loop is critical for FAK catalytic activity.
• FAK phosphorylates downstream substrates like NEDD9 (PubMed:9360983) and SRC to promote signaling complex assembly. Related references: PMID:9360983
• Ubiquitous expression; detected in B/T lymphocytes, lung fibroblasts, and epithelial cells (PubMed:31630787). Related references: PMID:31630787

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide spanning Tyr576/Tyr577; antibody specificity depends on phosphorylation state.
• Include phosphatase inhibitors in lysis buffers to preserve phospho-epitopes during sample preparation.
• For Western blot, a major band at ~119 kDa is expected; additional bands may represent degradation or cross-reactivity.
• Validate detection in activated cell lines (e.g., integrin-stimulated fibroblasts) where FAK Y576/577 phosphorylation is induced.
• For ELISA, consider coating with the phosphopeptide immunogen or using whole-cell lysates; include non‑phosphorylated peptide as negative control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one‑stop resource for IVD raw materials, offering validated antibody pairs and optimized monoclonal/polyclonal antibodies such as this anti‑phospho‑FAK (Y576/577) antibody for focal adhesion kinase research. We support procurement of bulk ancillary reagents and rare‑target raw materials, helping you streamline assay development from concept to clinical application.