Phospho-FADD-S194 Rabbit pAb for WB, ELISA - Q13158

Core Product Specifications and Parameters

Biological Background: FADD Function and Localization

• FADD (FAS-associated death domain protein), also known as MORT1 and GIG3, is a key adapter molecule in apoptotic signaling.
• Upon death receptor (e.g., FAS/CD95, TNFR1) activation, FADD recruits procaspases CASP8 or CASP10 to form the death-inducing signaling complex (DISC), leading to CASP8 proteolytic activation and initiation of the caspase cascade. Related references: PMID:16762833 PMID:19118384 PMID:20935634
• FADD participates in innate immunity by positively regulating interferon signaling and contributing to the antiviral immune response. Related references: PMID:21109225 PMID:24204270
• Localizes primarily to the cytoplasm.
• Widely expressed in various tissues, with low or absent expression in peripheral blood mononuclear leukocytes.
• Classified as a glycoprotein and phosphoprotein; undergoes phosphorylation that may regulate its activity.
• Predicted molecular weight approximately 23.3 kDa (UniProt Q13158).

Experimental Guidance and Technical Tips

• For Western blot, consider using a phospho‑specific lysis buffer and including phosphatase inhibitors to preserve phosphorylation.
• Verify specificity by treating samples with lambda phosphatase or by blocking with the non‑phosphorylated peptide.
• In ELISA, ensure the coating antigen contains the phosphorylated S194 epitope and use a phospho‑specific detection protocol.
• Validate the antibody in a relevant biological model where FADD phosphorylation is induced (e.g., apoptosis stimulation) and always include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with a reliable, one‑stop supply of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. With expertise in apoptosis and innate immunity targets, we can source rare raw materials such as phospho‑specific antibodies like this anti‑phospho‑FADD‑S194, supporting your projects from concept to clinic.