Phospho-ESRα-S167 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P03372

Core Product Specifications and Parameters

Biological Background: Estrogen Receptor Alpha Function and Localization

• Estrogen receptor alpha (ER-alpha), encoded by the ESR1 gene, is a steroid hormone receptor and member of the nuclear receptor subfamily 3 group A (NR3A1). It functions as a ligand-activated transcription factor, regulating gene expression critical for cell proliferation and differentiation in target tissues.
• Upon estrogen binding, ER-alpha undergoes a conformational change that facilitates homodimerization and binding to estrogen response elements (EREs) in DNA. It also interacts with other transcription factors such as AP-1 (c-Jun/c-Fos) and Sp1 to mediate ERE-independent signaling. Coactivator complexes are recruited via LXXLL motifs.
• Alternative splicing and promoter usage produce isoforms that lack one or more functional domains. These isoforms can modulate the transcriptional activity of the full-length receptor by competitive DNA or ligand binding, or by heterodimerization. Related references: PMID:10970861
• ER-alpha shuttles between the nucleus, cytoplasm, and cell membrane. It is also associated with the Golgi apparatus, enabling both genomic and rapid non-genomic signaling pathways, including activation of kinase cascades.
• The receptor is subject to multiple post-translational modifications including phosphorylation, glycosylation, palmitoylation, and ubiquitin-like conjugation. It contains zinc finger motifs and a ligand-binding domain, with a molecular weight of approximately 66 kDa.
• ER-alpha is widely expressed in human tissues but is notably absent in the pituitary gland. Related references: PMID:10970861
• Specific functional roles include maintaining neuronal survival under ischemic reperfusion injury in the presence of estradiol, and activating NOS3 to promote endothelial nitric oxide production. Related references: PMID:21937726
• ER-alpha also participates in mutual transrepression with NF-kappa-B, modulating inflammatory responses. Additionally, it mediates MTA1-dependent transcriptional regulation of BRCA1 and BCAS3. Related references: PMID:17922032

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic phosphorylated peptide around Ser167. Use phosphatase inhibitors during sample preparation to preserve phosphorylation.
• For western blotting, the predicted band size is ~66 kDa. Validate detection in human cell lines known to express ER-alpha, such as MCF-7.
• In immunofluorescence/ICC, anticipate nuclear, cytoplasmic, and membrane localization. Co-stain with compartment markers to confirm specificity.
• As a polyclonal antibody, lot-to-lot consistency may vary; include positive and negative controls, and consider a blocking peptide for phospho-specificity verification.
• While the antibody is reactive with human samples, cross-reactivity with other species has not been tested; empirical validation is recommended if used orthologously.
• For ELISA, a phosphorylated peptide coating antigen may help calibrate detection sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This phospho-specific ER-alpha antibody supports research and assay development for hormone-dependent cancers and estrogen signaling pathways. We offer reliable supply chains for validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets—helping you accelerate your diagnostic programs.