Phospho-ERK3-S189 Rabbit Polyclonal Antibody for WB, ELISA - Q16659

Core Product Specifications and Parameters

Biological Background: Mitogen-activated protein kinase 6 Function and Localization

• Mitogen-activated protein kinase 6 (MAPK6), also known as extracellular signal-regulated kinase 3 (ERK-3) or p97-MAPK, is an atypical MAPK encoded by the MAPK6 gene.
• MAPK6 lacks the typical TXY activation motif and displays low basal kinase activity; it phosphorylates substrates such as microtubule-associated protein 2 (MAP2) and MAPK-activated protein kinase 5 (MAPKAPK5).
• The kinase forms a complex with MAPKAPK5, where ERK3/MAPK6 is first phosphorylated at Ser-189, which then mediates phosphorylation and activation of MAPKAPK5; MAPKAPK5 in turn phosphorylates ERK3/MAPK6 in a regulatory feedback loop (By similarity).
• May play a role in promoting cell cycle entry, although the precise biological function remains under investigation (By similarity).
• Subcellularly, MAPK6 localizes to both the cytoplasm and nucleus, consistent with its involvement in signaling pathways that transmit extracellular cues.
• Tissue expression is highest in skeletal muscle and brain, with additional presence in heart, placenta, lung, liver, pancreas, kidney, and skin fibroblasts.
• Keywords from UniProt highlight its classification as a serine/threonine-protein kinase, nucleotide-binding, ATP-binding, phosphoprotein, and its involvement in the cell cycle.

Experimental Guidance and Technical Tips

• As a phospho-specific antibody targeting ERK3 phosphorylated at Ser-189, it is critical to use appropriate phosphatase inhibitors during sample preparation to preserve the phosphorylation signal.
• For Western blot (WB), consider including a phosphatase-treated control or a non-phospho peptide blocking experiment to confirm phospho-specificity.
• The antibody is reactive with human, mouse, and rat ERK3; validate detection in the relevant cell or tissue lysates where ERK3 is expressed (e.g., skeletal muscle, brain).
• For ELISA, use a phosphorylated peptide corresponding to the immunogen as a positive control and a non-phosphorylated peptide as a specificity control.
• Note that the predicted protein weight is approximately 83 kDa; however, the observed band may shift slightly depending on post-translational modifications or gel conditions.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to accelerate your diagnostic development.
• Whether you are developing assays targeting MAP kinase signaling pathways or need rare-target raw-material sourcing for phospho-specific antibodies, CamelBio provides reliable supply-chain solutions from concept to clinic.
• As your sourcing partner, we ensure seamless access to research-grade and IVD-grade antibodies, helping you maintain consistent performance across batches and applications.