Phospho-ErbB4/HER4-Y1284 Rabbit pAb - Q15303

Core Product Specifications and Parameters

Biological Background: ErbB4/HER4 Function and Localization

• ErbB4 (HER4) is a receptor tyrosine-protein kinase that serves as a cell surface receptor for neuregulins and EGF family ligands, playing critical roles in heart, CNS, and mammary gland development.
• The gene encodes multiple isoforms through alternative splicing (JM-A, JM-B, CYT-1, CYT-2), which differ in ligand specificity, signaling capacity, and proteolytic processing.
• Ligand binding induces receptor dimerization and autophosphorylation at specific tyrosines, including Y1284, creating docking sites for adaptor proteins to activate downstream pathways like MAPK and PI3K-AKT.
• Isoforms containing the CYT-1 domain (JM-A CYT-1, JM-B CYT-1) protect against apoptosis and promote cell migration via PI3K/AKT, while CYT-2 isoforms lack this activity.
• Proteolytic cleavage of JM-A isoforms releases a soluble intracellular domain (4ICD) that translocates to the nucleus to regulate transcription (e.g., milk protein genes via STAT5A) or to mitochondria to induce apoptosis.
• Subcellular localization includes the cell membrane, nucleus, and mitochondrion, reflecting its diverse signaling roles.
• ErbB4 is highly expressed in brain, heart, kidney, and mammary gland, with isoform-specific patterns; for example, JM-B isoforms predominate in the heart.
• It is involved in differentiation, proliferation, and apoptosis, and is associated with diseases like amyotrophic lateral sclerosis when mutated.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around tyrosine 1284, so the antibody specifically recognizes phosphorylated ErbB4 at this site. For WB, consider using phosphatase inhibitors during lysis to preserve phosphorylation status.
• Given applications include WB and ELISA, verify specificity by testing non-phosphorylated peptide competition or treating samples with phosphatases to confirm signal loss.
• ErbB4 is expressed in multiple tissues; validate in a relevant sample system (e.g., heart or brain lysates for endogenous detection, or overexpressed models for low-expressing cells).

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