Phospho-eNOS-S1177 Rabbit pAb for WB, ELISA - P29474

Core Product Specifications and Parameters

Biological Background: eNOS Function and Localization

• Nitric oxide synthase 3 (eNOS) is an endothelial-specific enzyme that catalyzes the production of nitric oxide (NO), a key signaling molecule in the cardiovascular system.
• eNOS-derived NO induces vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway, playing a critical role in blood pressure regulation. Related references: PMID:1378832
• NO generated by eNOS also mediates VEGF-induced angiogenesis in coronary vessels and promotes platelet activation, contributing to hemostasis.
• An alternatively spliced isoform of eNOS lacks catalytic activity and may act as a dominant-negative regulator by forming heterodimers with the active full-length enzyme.
• eNOS localizes to the cell membrane, caveolae, cytoplasm, cytoskeleton, and Golgi apparatus; membrane targeting is facilitated by N-terminal myristoylation and palmitoylation.
• Expression has been detected in platelets, placenta, liver, and kidney.
• eNOS is a phosphoprotein whose activity is modulated by phosphorylation, calcium/calmodulin binding, and cofactors such as FAD, FMN, heme, iron, zinc, and NADP.

Experimental Guidance and Technical Tips

• When performing Western blot, include phosphatase inhibitors in the lysis buffer to preserve phosphorylation signals, and optimize blocking and washing conditions for phospho-specific antibodies.
• For detection of eNOS phosphorylation at Ser1177, stimulation of endothelial cells with VEGF or exposure to shear stress may be employed; however, the response should be validated in the relevant sample system.
• In ELISA, ensure that the coating antigen or capture antibody is appropriately selected, and perform a titration to determine optimal working concentrations.
• As eNOS shares homology with other NOS isoforms, cross-reactivity should be assessed if using in systems with mixed isoform expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support diagnostic development. This phospho-eNOS-S1177 antibody is part of our portfolio targeting key signaling molecules in cardiovascular and endothelial biology. We assist manufacturers and researchers in securing reliable raw materials from early-stage development through clinical application.