Phospho-EIF4G1-S1108 Rabbit pAb for WB, ELISA - Q04637

Core Product Specifications and Parameters

Biological Background: EIF4G1 Function and Localization

• EIF4G1 (eukaryotic translation initiation factor 4 gamma 1, also known as p220) is a core scaffold component of the eIF4F complex, essential for cap-dependent translation initiation.
• It mediates mRNA cap recognition, ATP-dependent unwinding of 5'-terminal secondary structures, and ribosome recruitment (PubMed:29987188). Related references: PMID:29987188
• EIF4G1 exists in two mutually exclusive subcomplexes: with EIF1 to promote leaky scanning and avoid cap-proximal start codons, and with EIF4E to locate the start codon via a TISU element without scanning (PubMed:29987188). Related references: PMID:29987188
• Required for endoplasmic reticulum stress-induced ATF4 mRNA translation as part of the eIF4F complex (PubMed:29062139). Related references: PMID:29062139
• Localizes predominantly to the cytoplasm and can be recruited to stress granules upon cellular stress; also detected in the nucleus.
• Phosphorylation at Ser1108 and other residues, along with acetylation and methylation, regulates its activity, complex assembly, and interaction network.
• Mutations in EIF4G1 are linked to autosomal dominant Parkinson disease 18 (PARK18), implicating translation dysregulation in neurodegeneration.
• Functions in host-virus interactions by facilitating ribosomal shunting on viral mRNAs, a mechanism hijacked by certain viruses.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide targeting Ser1108. Confirm phosphorylation specificity by using phosphatase-treated lysates or non-phospho-specific controls.
• Predicted molecular weight is 175 kDa. For Western blot, use a low-percentage SDS-PAGE gel (e.g., 6–8%) and optimize transfer conditions for large proteins.
• Validated applications: Western blot and ELISA. When performing WB, consider a primary antibody dilution series to balance signal intensity and background.
• Cross-reactivity has been verified for human, mouse, and rat samples. For other species, validate expression levels and sequence homology before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, technical services, and consulting. This phospho-EIF4G1 (Ser1108) antibody is part of our comprehensive portfolio for translation regulation and neurodegeneration research, supported by validated antibody pairs and bulk ancillary reagents. For rare target or custom antibody needs, our team bridges your project from concept to clinic.