Phospho-eIF4EBP1-T37/T46 Rabbit mAb for WB and ELISA - Q13541

Core Product Specifications and Parameters

Biological Background: eIF4EBP1 Function and Localization

• eIF4EBP1 (Eukaryotic translation initiation factor 4E-binding protein 1), also known as PHAS-I, is a key repressor of translation initiation.
• It regulates EIF4E activity by competing with EIF4G1/EIF4G3 for binding to EIF4E. In its hypophosphorylated state, it tightly binds EIF4E and inhibits cap-dependent translation. Hyperphosphorylation, in response to growth signals, causes dissociation from EIF4E, allowing assembly of the eIF4F complex and translation initiation.
• Functions as a downstream effector of the mTORC1 and MAP kinase pathways, mediating protein synthesis in response to hormones, growth factors, and nutrients.
• Localizes to both cytoplasm and nucleus, consistent with its role in modulating translation and possibly nuclear functions.
• Undergoes extensive post-translational modifications including phosphorylation (e.g., at T37, T46, T70, S65), acetylation, and ubiquitination (noted as isopeptide bond and Ubl conjugation), which collectively regulate its activity and stability.
• Molecular weight approximately 13 kDa (12580 Da) as determined by sequence, though migration on SDS-PAGE may vary due to phosphorylation states.
• Classified as a protein synthesis inhibitor, phosphoprotein, and belongs to the translation regulation family; also identified in proteomics studies as a reference proteome component.

Experimental Guidance and Technical Tips

• This antibody recognizes endogenous levels of eIF4EBP1 only when phosphorylated at Thr37 and Thr46.
• For Western blot, include phosphatase inhibitors in lysis buffers to preserve phosphorylation signals. The major band typically migrates around 13-20 kDa; multiple bands may appear due to differential phosphorylation.
• Validate phospho-specific signal by treating cells with stimuli (e.g., insulin, serum) to induce phosphorylation, and optionally use phosphatase treatment or competition with non-phosphorylated peptide.
• For ELISA, optimize coating conditions and antibody concentration; consider pairing with a total eIF4EBP1 antibody for normalization.
• Cross-reactivity has been demonstrated in human and rat; for mouse, despite sequence homology, reactivity is not listed in cross-reactivity data; validation in mouse samples may be required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with a comprehensive portfolio of IVD raw materials, technical services, and consulting. This anti-phospho-eIF4EBP1 (T37/T46) antibody exemplifies our commitment to supplying validated phospho-specific detection reagents for mTOR pathway research—backed by our capability to source optimized monoclonal antibodies, recombinant proteins, and ancillary reagents for assay development. Whether you need bulk quantities or customized solutions, CamelBio bridges the gap from concept to clinic.