Phospho-eIF4EBP1 (T37/T46) Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13541

Core Product Specifications and Parameters

Biological Background: eIF4EBP1 Function and Localization

• eIF4EBP1, also known as PHAS-I or 4E-BP1, is a translation repressor that binds to the mRNA cap-binding protein eIF4E.
• In its hypophosphorylated state, eIF4EBP1 competitively inhibits the interaction between eIF4E and eIF4G, thereby preventing the assembly of the eIF4F translation initiation complex.
• Hyperphosphorylation of eIF4EBP1, triggered by growth factors and hormones via MAP kinase and mTORC1 pathways, causes its dissociation from eIF4E, allowing cap-dependent translation to proceed.
• eIF4EBP1 is a key mediator of translational control in response to extracellular stimuli, integrating signals that regulate cell growth and proliferation.
• The protein localizes to both the cytoplasm and nucleus, where it may play roles in nuclear export of eIF4E or nuclear translation.
• eIF4EBP1 undergoes multiple post-translational modifications, including phosphorylation at several sites (e.g., T37, T46), acetylation, and ubiquitin-like conjugation, which fine-tune its activity.
• It belongs to a family of eIF4E-binding proteins that share a conserved eIF4E-binding motif.

Experimental Guidance and Technical Tips

• Because the antibody targets phosphorylation at T37/T46, include phosphatase inhibitors in cell/tissue lysis buffers to maintain the phosphorylation signal.
• For Western blotting, the expected molecular weight of eIF4EBP1 is approximately 13 kDa, but multiple bands corresponding to different phosphorylation states may be observed.
• For immunohistochemistry and immunofluorescence, optimize antigen retrieval methods and antibody dilution for your specific sample type.
• Validate the antibody’s specificity using appropriate positive controls (e.g., cells treated with insulin or serum) and negative controls (e.g., phosphatase-treated lysates or non-phosphorylated peptide blocking).
• The antibody is suitable for multiple applications (WB, IHC-P, IF/ICC, ELISA); consider the recommended detection ranges for each technique.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, from validated antibodies and antigen pairs to bulk ancillary reagents. Our one-stop sourcing channel simplifies procurement for phospho-signaling research targets like eIF4EBP1, helping you accelerate immunoassay development and translational studies. Whether you need rare target antibodies or production-scale quantities, CamelBio is your reliable partner from concept to clinic.