Phospho-eIF4E-S209 Rabbit pAb - P06730

Core Product Specifications and Parameters

Biological Background: eIF4E Function and Localization

• Eukaryotic translation initiation factor 4E (eIF4E), also known as eIF-4F 25 kDa subunit and mRNA cap-binding protein, is a key regulator of protein synthesis.
• Recognizes and binds the 7-methylguanosine (m7G) cap of mRNA, inducing unwinding of secondary structures to facilitate ribosome binding during translation initiation (PubMed:16271312, PubMed:22578813).
  Related references: PMID:16271312 PMID:22578813
• Acts in the nucleus to promote export of a subset of mRNAs, stimulating RNA capping, processing, and splicing (PubMed:11606200, PubMed:22578813, PubMed:22684010).
  Related references: PMID:11606200 PMID:22578813 PMID:22684010
• Together with EIF4G1, antagonizes ribosomal scanning and is required for TISU (Translation Initiator of Short 5' UTR) element-dependent translation, which bypasses scanning (PubMed:29987188).
  Related references: PMID:29987188
• In the cytoplasm, regulates translation and mRNA stability; in P-bodies, mediates storage of translationally inactive mRNAs, preventing their degradation (PubMed:24335285).
  Related references: PMID:24335285
• Promotes nuclear export of specific mRNAs, including NOS2/iNOS (PubMed:23471078) and MDM2 (PubMed:22684010), influencing immune responses and cell cycle control.
  Related references: PMID:23471078 PMID:22684010
• Localizes to the cytoplasm, P-bodies, stress granules, nucleus, nuclear speckles, and nuclear bodies, reflecting its multifaceted roles.
• Post-translationally modified by phosphorylation and acetylation; dysregulation of eIF4E is linked to autism spectrum disorders and exploited by viruses for protein synthesis.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around S209; verify specificity with phosphatase treatment or non-phosphorylated peptide competition in your assay system.
• For Western blotting, the predicted molecular weight is ~25 kDa; optimize electrophoresis and transfer conditions, and consider using low-fluorescence membranes to reduce background.
• In ELISA, pair this antibody with a total eIF4E antibody to quantify phosphorylation levels; validate linearity and sensitivity in your sample matrix.
• Cross-reactivity is reported for human and mouse; test on lysates from your species of interest and adjust antibody dilution accordingly.
• For phospho-specific detection, ensure samples are prepared with phosphatase inhibitors to preserve the modification state.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, empowering diagnostic manufacturers and researchers from concept to clinic. This anti-phospho-eIF4E-S209 antibody supports your investigations into translational control, mRNA export, and associated pathologies. Explore our portfolio of validated antibody pairs, bulk reagents, and custom sourcing solutions to accelerate your assay development.