Phospho-eIF2α-S49 Rabbit Polyclonal Antibody for WB, IHC-P - P05198

Core Product Specifications and Parameters

Biological Background: eIF2α (EIF2S1) Function and Localization

• EIF2S1 encodes the alpha subunit of the eukaryotic translation initiation factor 2 (eIF2), a heterotrimer that delivers initiator Met-tRNAi to the 40S ribosomal subunit. Related references: PMID:16289705
• eIF2, together with GTP and Met-tRNAi, forms a ternary complex that binds the 40S subunit to generate the 43S pre-initiation complex; subsequent mRNA recruitment leads to 80S complex formation upon GTP hydrolysis. Related references: PMID:16289705
• To reactivate eIF2, the GDP must be exchanged for GTP by the guanine nucleotide exchange factor eIF2B. Related references: PMID:16289705
• Phosphorylation at Ser49 (and other serine residues) by stress‑sensing kinases (EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK, EIF2AK4/GCN2) converts eIF2α into a global translation inhibitor, attenuating cap‑dependent protein synthesis while favoring translation of stress‑responsive mRNAs such as ATF4 and QRICH1. Related references: PMID:19131336 PMID:33384352 PMID:38340717
• eIF2α also participates in mitophagy: upon mitochondrial damage, phospho‑eIF2α relocalizes to the mitochondrial surface to promote PRKN‑independent mitophagy. Related references: PMID:38340717
• Subcellular localization includes the cytoplasm, cytosol, stress granules, and mitochondria.
• Post‑translational modifications include acetylation and extensive phosphorylation; eIF2α binds RNA and is classified as an initiation factor, phosphoprotein, and reference proteome member.

Experimental Guidance and Technical Tips

• The immunogen is a phospho‑specific peptide corresponding to residues surrounding Ser49 of human EIF2S1. To preserve phosphorylation, include phosphatase inhibitors in lysis buffers and work quickly.
• For Western blot, a band at approximately 36 kDa is expected. Confirm specificity by treating samples with λ‑phosphatase or using non‑phospho‑eIF2α antibodies in parallel.
• Reported cross‑reactivity with mouse and rat allows multi‑species studies; validate in the relevant sample system.
• For IHC‑P, optimize antigen retrieval (e.g., citrate buffer, pH 6.0) and primary antibody dilution. Include positive and negative control tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting. This phospho‑eIF2α antibody supports research into the integrated stress response and translational control; we also supply matched detection antibodies, bulk ancillary reagents, and can source rare‑target raw materials to accelerate your assay development. Let CamelBio bridge your concept‑to‑clinic journey.