Phospho-EGFR-Y1197 Rabbit pAb for WB - P00533

Core Product Specifications and Parameters

Biological Background: Epidermal Growth Factor Receptor (EGFR) Function and Localization

• EGFR is a receptor tyrosine kinase that binds EGF family ligands and activates multiple signaling cascades, governing cell growth, survival, and differentiation. Related references: PMID:10805725 PMID:27153536 PMID:2790960
• Ligand-induced dimerization leads to autophosphorylation on key tyrosines; phosphorylation at Y1197 creates a docking site for GRB2. Related references: PMID:27153536
• Activated EGFR triggers at least four major pathways: RAS-RAF-MEK-ERK, PI3K-AKT, PLCγ-PKC, and STATs. Related references: PMID:27153536
• EGFR directly phosphorylates substrates like RGS16 and MUC1, modulating G protein signaling and cell adhesion. Related references: PMID:11602604 PMID:11483589
• Interaction with CCDC88A/GIV promotes sustained membrane retention and cell migration. Related references: PMID:20462955
• An alternative isoform (isoform 2) may act as an antagonist of EGF signaling.
• EGFR is predominantly at the plasma membrane, but also traffics to endosomes, Golgi, endoplasmic reticulum, and nucleus; a secreted form exists. Ubiquitously expressed, with isoform 2 also in ovarian cancers.

Experimental Guidance and Technical Tips

• For Western blotting, use phosphatase inhibitor cocktails during cell lysis to preserve phosphorylation at Y1197.
• Validate antibody specificity by comparing untreated versus EGF-stimulated cell lysates; a substantial increase in band intensity around 134 kDa is expected upon activation.
• The mature EGFR protein has a predicted molecular weight of ~134 kDa, but post-translational modifications such as glycosylation may cause a mobility shift; use appropriate molecular weight markers and positive controls.
• As a general starting point for phospho-specific polyclonal antibodies, consider testing dilutions between 1:500 and 1:2000; optimize for your specific experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibodies, antigens, and ancillary reagents, supporting diagnostic manufacturers and research laboratories. For EGFR signaling research, we offer high-quality phospho-specific antibodies like this anti-EGFR Y1197 reagent, with bulk ordering options and customized sourcing for rare targets. Partner with CamelBio to streamline your raw material procurement from concept to clinical application.