Phospho-EGFR-Y1045 Rabbit pAb for WB, ELISA - P00533

Core Product Specifications and Parameters

Biological Background: EGFR Function and Localization

• Epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase that binds ligands of the EGF family, including EGF, TGF-alpha, AREG, BTC, EREG, and HBEGF, to trigger downstream signaling. Related references: PMID:12297049, PMID:15611079, PMID:17909029
• Ligand binding induces receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic tyrosine residues, such as Y1045, creating docking sites for adapter proteins like GRB2 and activating cascades including RAS-RAF-MEK-ERK, PI3K-AKT, PLCgamma-PKC, and STATs. Related references: PMID:27153536
• EGFR directly phosphorylates substrates like RGS16 and MUC1, and regulates cell migration, learning, memory, and pain signaling. Related references: PMID:11602604, PMID:11483589, PMID:20462955
• Isoform 2 may act as an antagonist of EGF action and is notably expressed in ovarian cancers.
• Subcellular localization includes the plasma membrane, endosomes, Golgi apparatus, endoplasmic reticulum, nucleus, and secreted forms.
• Post-translational modifications encompass phosphorylation, glycosylation, ubiquitination, and palmitoylation, as highlighted by keywords such as Phosphoprotein, Glycoprotein, and Lipoprotein.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Y1045; use phosphatase inhibitors during sample preparation to preserve phosphorylation.
• For Western blot, consider loading EGF-stimulated versus unstimulated cell lysates as positive and negative controls, and titrate the antibody for optimal signal.
• Direct ELISA can be performed; evaluate coating and detection conditions empirically for your specific assay format.
• Cross-reactivity has been demonstrated with human EGFR; for other species, validate due to potential sequence differences.
• Confirm specificity by peptide competition or phosphatase treatment in relevant biological samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this Phospho-EGFR-Y1045 antibody as part of our comprehensive portfolio of IVD raw materials, supporting diagnostic manufacturers and researchers in oncology and signal transduction. Beyond individual antibodies, we provide validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and rare target sourcing to streamline assay development. Partner with CamelBio to efficiently bridge the gap from concept to clinic, with reliable access to the critical raw materials you need.