Phospho-EEF2K-S366 Rabbit Polyclonal Antibody for WB, ELISA - O00418

Core Product Specifications and Parameters

Biological Background: EEF2K Function and Localization

• EEF2K is a threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation.
• Upon activation by upstream kinases such as AMPK or TRPM7, it phosphorylates elongation factor EEF2 at a single site, rendering EEF2 unable to bind ribosomes and thus reducing protein synthesis.
• It belongs to the serine/threonine protein kinase family and is also referred to as calcium/calmodulin-dependent kinase III (CaMKIII).
• As a calmodulin-binding kinase, its activity is dependent on calcium and calmodulin, and it contains an ATP-binding nucleotide-binding domain.
• EEF2K is classified as a phosphoprotein and its function is influenced by multiple upstream phosphorylation events.
• Keywords associated with this protein include ATP-binding, calcium, calmodulin-binding, kinase, nucleotide-binding, phosphoprotein, and transferase.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around S366; it may preferentially detect EEF2K phosphorylated at Ser366, while reactivity with the non-phosphorylated form could be limited.
• For Western blotting, include phosphatase inhibitors in lysis buffers and consider using a phospho-specific detection protocol to preserve phosphorylation.
• The predicted molecular weight is 82 kDa; select gel percentage and transfer conditions accordingly, and verify the band size in your experimental system.
• The antibody shows cross-reactivity with human, mouse, and rat, indicating sequence conservation; always validate performance in your target species and tissue type.
• In ELISA applications, the synthetic phosphopeptide antigen may serve as a positive control for optimizing coating and detection conditions.
• As a polyclonal antibody, lot-to-lot consistency may vary; we recommend titrating and validating each new lot before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and academic institutes with a single access point to validated IVD raw materials. This phospho‑specific EEF2K antibody supports studies in translational control and kinase signaling, and we can supply it alongside matched antibody pairs, bulk ancillary reagents, and custom sourcing for rare‑target biomaterials. Let CamelBio streamline your procurement pipeline and accelerate progress from concept to clinic.