Phospho-DRP1-S616 Rabbit mAb - O00429

Core Product Specifications and Parameters

Biological Background: DRP1 Function and Localization

• Dynamin-1-like protein (DNM1L), commonly referred to as DRP1, is a member of the dynamin superfamily of large GTPases and is also known as DLP1, DVLP, and Dymple.
• DRP1 mediates mitochondrial and peroxisomal fission by oligomerizing into membrane-associated tubular structures that wrap around scission sites, constricting and severing membranes through a GTP hydrolysis-dependent mechanism. Related references: PMID:11514614, PMID:12499366, PMID:17301055, PMID:23530241, PMID:23584531, PMID:33850055
• Recruitment to mitochondrial membranes is driven by receptors such as MFF, MIEF1, and MIEF2 in a GTP-dependent manner; subsequent GTP hydrolysis releases DRP1 and allows filament rings to close and sever the double membrane. Related references: PMID:23283981, PMID:23921378, PMID:29899447
• DRP1 promotes mitochondrial fission during mitosis and acts downstream of PINK1 in a PRKN-dependent pathway, and its rhythmic phosphorylation at Ser-637 controls circadian ATP production. Related references: PMID:19411255, PMID:26992161, PMID:27301544, PMID:29478834, PMID:32484300
• DRP1 is required for normal brain development, including the cerebellum, and is essential for postmitotic neuronal survival by suppressing oxidative damage; it also facilitates developmental apoptosis during neural tube formation. Related references: PMID:17460227, PMID:26992161, PMID:27145208
• Ubiquitously expressed with highest levels in skeletal muscle, heart, kidney, and brain; multiple isoforms show tissue-specific patterns, including a brain-specific isoform 1 and testis/skeletal muscle-enriched isoforms 2 and 3.
• Subcellular localization includes the mitochondrial outer membrane, peroxisomes, cytosol, Golgi apparatus, clathrin-coated pits, and synaptic vesicle membranes; DRP1 is subject to extensive post-translational modifications, including phosphorylation, acetylation, S-nitrosylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to residues around Ser-616 of human DRP1. The antibody is expected to specifically recognize DRP1 only when phosphorylated at this site.
• For western blotting, consider using phosphatase inhibitors during sample preparation to preserve the S616 phosphorylation signal, and validate the band at the predicted molecular weight of 82 kDa in your experimental system.
• In immunoprecipitation, titrate the antibody amount and use optimized lysis buffers to maintain the phospho-epitope; confirm specificity through phosphatase treatment controls.
• For ELISA applications, including competitive peptide assays, the antibody’s reactivity should be verified against the phosphorylated vs. non-phosphorylated peptide to ensure phospho-specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with one-stop access to premium IVD raw materials, including validated antibodies, antigens, and ancillary reagents. This phospho-specific DRP1 antibody supports mitochondrial dynamics research and related assay development. Our comprehensive supply network covers rare-target sourcing, bulk orders, and technical consultation, streamlining your development pipeline from concept to clinic.