Phospho-DOK2-Y299 Rabbit pAb - O60496

Core Product Specifications and Parameters

Biological Background: Docking protein 2 Function and Localization

• DOK2, also known as p56(dok-2) or downstream of tyrosine kinase 2, is a member of the docking protein family of adaptor/scaffold proteins that lack enzymatic activity.
• It functions as a docking platform for the assembly of multimolecular signaling complexes, modulating cellular proliferation induced by cytokines such as IL-4, IL-2, and IL-3.
• DOK2 is involved in modulating Bcr-Abl signaling pathways, potentially influencing leukemogenic processes.
• It attenuates EGF-stimulated MAP kinase activation, acting as a negative regulator of growth factor receptor signaling.
• The protein is highly expressed in peripheral blood leukocytes, lymph nodes, and spleen, with lower expression in thymus, bone marrow, and fetal liver.
• DOK2 undergoes phosphorylation, particularly at tyrosine residues, which is critical for its adapter function in signal transduction.
• It has been identified as a host-virus interaction factor and is a reference proteome protein with available 3D structural data.

Experimental Guidance and Technical Tips

• The immunogen is a phospho-specific peptide surrounding Y299 of human DOK2, indicating that this antibody recognizes the phosphorylated form of the protein.
• For Western blot, consider using phospho-specific controls or phosphatase treatment to confirm detection specificity.
• In immunofluorescence/ICC, validate antibody binding by peptide competition or by comparing signal in stimulated vs. unstimulated cells.
• Due to the conserved nature of DOK2 phosphorylation sites, verify cross-reactivity in non-human species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering a comprehensive portfolio of validated antibodies, including phosphorylation-specific reagents for signal transduction research. For DOK2 and related targets, we can supply matched antibody pairs, bulk monoclonal and polyclonal antibodies, and ancillary reagents to support assay development. Contact us to explore custom sourcing solutions for rare or specialized raw materials.