Phospho-Cyclin E1-T395 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P24864

Core Product Specifications and Parameters

Biological Background: Cyclin E1 Function and Localization

• Cyclin E1 is a G1/S-specific cyclin essential for the control of the cell cycle at the G1/S transition. It complexes with and activates cyclin-dependent kinase 2 (CDK2), driving progression from G1 to S phase.
• It belongs to the cyclin family, a group of regulatory proteins that oscillate in concentration during the cell cycle and govern key checkpoints.
• The protein localizes predominantly to the nucleus, consistent with its role in regulating transcription and cell cycle machinery.
• Tissue expression is highest in testis and placenta, with low levels in bronchial epithelial cells, reflecting its function in proliferating tissues.
• Post-translational modifications include phosphorylation at multiple residues (such as T395), ubiquitination, and potential sumoylation, which modulate protein stability and activity.
• Alternative splicing generates isoforms with distinct regulation and possibly tissue-specific functions.
• The calculated molecular weight is approximately 47,077 Da (UniProt), consistent with the observed ~47 kDa band in western blotting.
• UniProt annotations highlight involvement in cell division, cyclin-dependent kinase regulation, and proteomics identification, underscoring its significance in normal and cancer cell proliferation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phospho-peptide surrounding T395, making this antibody ideal for specifically detecting phosphorylated Cyclin E1 at this residue. For confirming phospho-specificity, consider treating samples with λ-phosphatase or using non-phospho peptide blocking.
• In western blotting, a band around 47 kDa is expected; however, validate the molecular weight in your specific sample system, as post-translational modifications may shift migration.
• For immunofluorescence/immunocytochemistry, anticipate nuclear staining. Include DAPI or another nuclear counterstain to verify localization, and use appropriate fixation/permeabilization conditions to preserve phospho-epitopes.
• When applying to ELISA, the antibody may capture or detect phospho-Cyclin E1 in lysates; optimize coating and detection steps for your assay format.
• Cross-reactivity is reported for human, mouse, and rat; if using other species, perform sequence alignment around T395 and preliminary validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges diagnostic developers and researchers to premier IVD raw materials, including this phospho-specific Cyclin E1 antibody, which supports cell cycle and oncology research applications. As a comprehensive sourcing partner, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw materials—empowering your projects from concept to clinic with a streamlined, one-stop procurement experience.