Phospho-Cyclin D1 (T286) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P24385

Core Product Specifications and Parameters

Biological Background: Cyclin D1 Function and Localization

• Cyclin D1 (CCND1) is a G1/S-specific cyclin, also known as B-cell lymphoma 1 protein (BCL-1) and PRAD1 oncogene.
• It functions as the regulatory subunit of the cyclin D1-CDK4 complex, which phosphorylates retinoblastoma (RB) family proteins to release E2F transcription factors, thereby promoting G1/S transition. Related references: PMID:1827756, PMID:1833066, PMID:19412162
• Additionally, Cyclin D1 acts as a substrate for SMAD3, repressing SMAD3 transcriptional activity; forms a ternary complex with CDK4 and CDKN1B for nuclear import; and exerts corepressor activity with INSM1 on promoters independently of cell cycle. Related references: PMID:15241418, PMID:9106657, PMID:16569215, PMID:18417529
• Subcellularly, Cyclin D1 is found in the nucleus, cytoplasm, and nucleus membrane.
• Post-translational modifications: Cyclin D1 is a phosphoprotein, with documented isopeptide bond and ubiquitin-like conjugation features.
• Cyclin D1 is a key cell cycle regulator and proto-oncogene, implicated in cancer through chromosomal rearrangements.

Experimental Guidance and Technical Tips

• This antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Thr-286 of human Cyclin D1; consider its suitability for detecting endogenous phosphorylated Cyclin D1 in stimulated or cycling cells.
• For Western blotting, a major band around 34 kDa is expected; inclusion of phosphatase inhibitors in lysis buffer is recommended to preserve phosphorylation signals.
• In immunohistochemistry (IHC-P), optimal antigen retrieval and blocking conditions should be optimized; phospho-specificity can be confirmed by phosphatase treatment or phospho-blocking peptide competition.
• ELISA applications may require titration of antibody and antigen concentrations; validate assay specificity with non-phosphorylated peptide controls.
• Cross-reactivity is indicated for human, mouse, and rat samples; however, sequence alignment of the immunogen with target species is advised to confirm conservation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to provide reliable access to IVD raw materials, including validated phospho-specific antibodies like this anti-Phospho-Cyclin D1 (T286) rabbit polyclonal antibody. From recombinant proteins and antibody pairs to optimized monoclonal/polyclonal antibodies and bulk ancillary reagents, we offer a one-stop sourcing solution for your cell cycle and oncology projects. Contact us for custom rare-target raw-material procurement and technical consulting to accelerate your assay development from concept to clinic.