Phospho-Crk p38-Y221 Rabbit Monoclonal Antibody for WB, ELISA - P46108

Core Product Specifications and Parameters

Biological Background: Crk p38 Function and Localization

• Adapter molecule crk (also known as Proto-oncogene c-Crk or p38) is an SH2/SH3 domain-containing signaling adaptor encoded by the CRK gene.
• It plays a critical role in cell branching, adhesion, spreading, and migration through BCAR1-CRK-RAPGEF1 signaling, leading to RAP1 activation.
• Crk mediates attachment-induced MAPK8 activation, membrane ruffling, and cell motility in a Rac-dependent manner. Related references: PMID:31311869
• It is involved in phagocytosis of apoptotic cells and cell motility via interactions with DOCK1 and DOCK4. Related references: PMID:19004829
• Crk may also regulate EFNA5-EPHA3 signaling (By similarity).
• The protein localizes to both the cytoplasm and cell membrane.
• Post-translational modifications include phosphorylation at multiple sites, and it is subject to alternative splicing, producing isoforms such as Crk I and Crk II (CRKII).
• Conserved domains include an SH2 domain and two SH3 domains, which mediate protein-protein interactions in signaling pathways.

Experimental Guidance and Technical Tips

• For Western blot, consider using phosphatase-treated cell lysates or a non-phosphorylated peptide as a negative control to confirm phospho-specificity at Y221.
• In ELISA, compare signals from phosphorylated versus non-phosphorylated peptides to assess antibody specificity.
• The expected molecular weight of the target is approximately 34 kDa; verify the band size in your specific cell line or tissue sample.
• Optimize antibody dilution and incubation conditions in your assay system, as performance can vary with sample type and preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers investigating Crk-mediated signaling in cell adhesion and migration, we offer validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. Our sourcing services can also support your rare target raw-material needs.