Phospho-CREB1-S142 Rabbit pAb for WB - P16220

Core Product Specifications and Parameters

Biological Background: CREB1 Function and Localization

• CREB1 (Cyclic AMP-responsive element-binding protein 1), also known as CREB and CREB-1, is encoded by the CREB1 gene.
• It functions as a phosphorylation-dependent transcription factor that binds the cAMP response element (CRE) in target gene promoters; its activity is enhanced by TORC coactivators independently of Ser-119 phosphorylation (PubMed:14536081). Related references: PMID:14536081
• Involved in multiple cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells (By similarity).
• In cardiomyocytes, CREB1 regulates the expression of apoptotic and inflammatory response factors downstream of ERFE-mediated AKT signaling (By similarity).
• Subcellularly, CREB1 is predominantly localized in the nucleus, consistent with its transcriptional regulatory role.
• CREB1 is a phosphoprotein; phosphorylation at Ser142 (as recognized by this antibody) modulates its transcriptional activity and protein interactions.
• Additional features include alternative splicing, ubiquitination, and involvement in host-virus interactions, highlighting a broad functional repertoire.

Experimental Guidance and Technical Tips

• For Western blot detection of phospho-CREB1 (S142), use lysis buffer supplemented with phosphatase inhibitors to maintain phosphorylation status.
• Verify antibody specificity by comparing signals from stimulated and phosphatase-treated samples; a loss of signal upon dephosphorylation confirms phospho‑specificity.
• A predominant band near 35 kDa corresponds to phosphorylated CREB1; additional bands may arise from other modifications or isoforms.
• Cross‑reactivity with human, mouse, and rat is predicted; validate reactivity in your specific sample system before quantitative analyses.
• Optimize blocking and antibody incubation conditions, as phospho‑specific antibodies may require lower stringency for optimal detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high‑quality IVD raw materials, including the Phospho-CREB1-S142 Rabbit pAb. Our portfolio encompasses validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents tailored to immunodetection workflows. Count on our one‑stop sourcing model to efficiently bridge your assay development needs from concept to clinic.