Phospho-CREB1-S133 Rabbit pAb for WB, IHC-P, IF/ICC, ELISA - P16220

Core Product Specifications and Parameters

Biological Background: CREB1 Function and Localization

• CREB1 (cAMP-responsive element-binding protein 1) is a phosphorylation-dependent transcription factor that binds to cAMP response elements (CRE) in viral and cellular promoters, stimulating gene transcription.
• Its transcriptional activity is enhanced by TORC coactivators independently of Ser-119 phosphorylation (PubMed:14536081). Related references: PMID:14536081
• It plays key roles in diverse cellular processes, including circadian rhythm synchronization and adipocyte differentiation (by similarity).
• In cardiomyocytes, CREB1 regulates apoptotic and inflammatory response genes downstream of ERFE-mediated AKT signaling (by similarity).
• The protein localizes to the nucleus, consistent with its role in transcription regulation.
• Post-translational modifications include phosphorylation, ubiquitination (Ubl conjugation), and alternative splicing, enhancing functional diversity.
• It is implicated in chromosomal rearrangements linked to cancer and host-virus interactions, reflecting its broad biological significance.

Experimental Guidance and Technical Tips

• This antibody was raised against a phosphorylated peptide spanning Ser133; ensure that sample preparation preserves phosphorylation (e.g., include phosphatase inhibitors) to maintain the epitope.
• For Western blot, the predicted molecular weight is ~35kDa; confirm band specificity by treating samples with lambda phosphatase.
• Immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC) may require antigen retrieval optimized for phospho-specific antibodies; consider testing multiple retrieval conditions.
• ELISA can be employed for quantitative detection of phospho-CREB1; validate with known positive and negative controls in your system.
• Cross-reactivity with mouse and rat CREB1 is reported; sequence alignment shows high conservation around the phosphorylation site.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips diagnostic manufacturers and research laboratories with validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For transcription factor studies like CREB1, we provide comprehensive raw-material solutions to accelerate assay development from concept to clinical application.