Phospho-CREB1-S133 Rabbit Monoclonal Antibody for WB, IHC-P, FC (intra), ELISA - P16220

Core Product Specifications and Parameters

Biological Background: CREB1 Function and Localization

• Cyclic AMP-responsive element-binding protein 1 (CREB1) is a phosphorylation-dependent transcription factor that binds to the cAMP response element (CRE) in DNA.
• CREB1 stimulates transcription upon binding to CRE, a sequence present in many viral and cellular promoters; activation is enhanced by TORC coactivators acting independently of Ser-119 phosphorylation. Related references: PMID:14536081
• It is involved in diverse cellular processes, including the synchronization of circadian rhythmicity and the differentiation of adipose cells.
• In cardiomyocytes, CREB1 regulates the expression of apoptotic and inflammatory response factors in response to ERFE-mediated AKT signaling.
• The protein localizes exclusively to the nucleus, consistent with its role as a transcription factor.
• CREB1 is a phosphoprotein subject to alternative splicing and has been identified in chromosomal rearrangements, highlighting its regulatory complexity and disease relevance.

Experimental Guidance and Technical Tips

• This antibody detects a phosphorylation site; to preserve the signal, consider adding phosphatase inhibitors during sample preparation.
• For Western blotting, run parallel blots with a pan-CREB antibody to distinguish changes in phosphorylation from total protein levels.
• In IHC-P, certain antigen retrieval methods may dephosphorylate the epitope; optimize retrieval conditions (e.g., milder heat or enzymatic retrieval) to maintain reactivity.
• During intracellular flow cytometry, use fixation and permeabilization protocols designed to retain phosphorylated epitopes, and include appropriate isotype controls.
• ELISA formats should be validated in the relevant biological matrix; consider using a phospho-specific capture or detection approach for optimal sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for IVD manufacturers and researchers by providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. With a strong focus on transcription factors and signaling molecules such as CREB1, we support your assay development from concept to clinic. Whether you require rare-target raw materials or scalable supply, our one-stop platform simplifies the sourcing of diagnostic-grade reagents.