Phospho-CREB1-S129 Rabbit pAb - P16220-1

Core Product Specifications and Parameters

Biological Background: CREB1 Function and Localization

• CREB1, also referred to as CREB or CREB-1, stands for Cyclic AMP-responsive element-binding protein 1, a transcription factor central to cAMP-mediated signaling.
• The human CREB1 gene (GeneID: 1385) encodes multiple isoforms through alternative splicing; UniProt entry P16220-1 corresponds to isoform 2.
• Isoform 2 of CREB1 has a calculated molecular mass of 36688 Daltons, consistent with the ~36–37 kDa band observed in western blots.
• Phosphorylation at serine 129 is a key post-translational modification that can influence CREB1 transcriptional activity and its interaction with co-regulatory factors.

Experimental Guidance and Technical Tips

• To preserve labile phosphorylation during cell or tissue lysis, include phosphatase inhibitors and work on ice; avoid prolonged sample storage unless snap-frozen.
• For western blot, load 20–50 µg of total protein per lane and probe with the antibody after verifying transfer efficiency; a band near 36–37 kDa is expected for human and rat CREB1.
• In ELISA, consider a peptide competition assay (using the immunogen peptide vs. a non-phosphorylated peptide) to confirm phospho-specificity.
• Always validate the antibody in your experimental system with appropriate controls, such as phosphatase-treated samples or cell lines with known CREB1 activation states.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials and technical support—from concept to clinic. This phospho‑specific CREB1 antibody supports signal transduction and transcription regulation research, and we offer complementary validated antibody pairs, optimized monoclonal/polyclonal reagents, and bulk ancillary materials. For rare or custom targets, our sourcing network can help secure the raw materials you need.