Phospho-Connexin 43-S368 Rabbit Polyclonal Antibody for IHC-P, ELISA - P17302

Core Product Specifications and Parameters

Biological Background: Connexin-43 Function and Localization

• Encodes the gap junction protein connexin-43 (Cx43), a 43 kDa transmembrane protein essential for intercellular communication.
• Core function: Forms hexameric connexons that dock between adjacent cells to create gap junction channels, permitting direct exchange of ions, metabolites, and small molecules (<1 kDa). Mediates electrical and metabolic coupling in tissues like heart and brain.
• Gene family and aliases: The GJA1 gene product is also known as CX43, GJAL, HSS, CMDR, EKVP, and others; mutations link to diseases like oculodentodigital dysplasia (ODDD).
• Subcellular localization: Resides primarily at the plasma membrane in gap junction plaques; also localized to the endoplasmic reticulum–Golgi intermediate compartment, with rapid turnover.
• Tissue expression: Highly expressed in cardiac muscle (intercalated disks), fetal cochlea, and skin keratinocytes (PubMed:11741837, PubMed:18662195, PubMed:38375917, PubMed:26604139). Essential for heart morphogenesis and auditory potassium recycling. Related references: PMID:11741837 PMID:18662195 PMID:38375917 PMID:26604139
• Post-translational modifications: Cx43 is a heavily modified phosphoprotein (Keyword: Phosphoprotein); phosphorylation at serine residues like S368 modulates channel gating, assembly, and degradation. Also modified by acetylation, S-nitrosylation, and ubiquitination.
• Innate immune function: Acts as a conduit for intercellular innate immune signaling by translocating the second messengers 2',3'-cGAMP and 2'-5' oligoadenylates from virus-infected cells to bystander cells and macrophages, thereby amplifying antiviral immunity (PubMed:24077100, PubMed:31992625, PubMed:40010341). Related references: PMID:24077100 PMID:31992625 PMID:40010341

Experimental Guidance and Technical Tips

• Immunogen design: A synthetic phosphopeptide corresponding to residues around S368 of human GJA1 was used. This ensures specificity for the phosphorylated Ser-368 epitope; verify with phosphatase treatment or non-phosphopeptide blocking in your assay system.
• Recommended applications: Based on specifications, the antibody is suitable for IHC-P and ELISA. For IHC-P, consider antigen retrieval with citrate buffer (pH 6.0) and test a concentration range of 1:50–1:200 using formalin-fixed paraffin-embedded tissue sections known to express Cx43 (e.g., heart, skin). For ELISA, coat with phospho-peptide and use the antibody at 1:500–1:2000, with appropriate phospho-specificity controls.
• Cross-reactivity: Demonstrates reactivity with mouse and rat Cx43 due to high sequence conservation. Validate in your specific experimental model.
• Storage: Shipped on ice; upon receipt, store at -20°C (short term) or -80°C (long term) in aliquots to avoid freeze-thaw cycles. Avoid prolonged exposure to room temperature.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This phospho-specific connexin-43 antibody supports research into gap junction biology, cardiac disease, and innate immunity. Beyond this product, we offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to streamline your assay development and production workflows.