Phospho-Chk1-S317 Rabbit Polyclonal Antibody (WB, ELISA) - O14757

Core Product Specifications and Parameters

Biological Background: Chk1 Function and Localization

• Serine/threonine-protein kinase Chk1 (CHEK1) is a critical cell cycle checkpoint kinase that ensures genome integrity by mediating cell cycle arrest and DNA repair upon DNA damage or replication stress. Related references: PMID:11535615, PMID:12399544, PMID:12446774
• Chk1 phosphorylates CDC25A, CDC25B, and CDC25C, leading to their inhibition via 14-3-3 binding or proteasomal degradation, thereby preventing CDK-cyclin activation and blocking cell cycle progression. Related references: PMID:12676583, PMID:12676925, PMID:12759351, PMID:9278511, PMID:19734889, PMID:20090422
• It directly phosphorylates RAD51 at Thr-309 to promote homologous recombination repair, TP53 at multiple C-terminal sites to enhance its activation and cell cycle arrest, and FANCE to facilitate DNA cross-link repair. Related references: PMID:15665856, PMID:10673501, PMID:15659650, PMID:16511572, PMID:17296736
• Chk1 phosphorylates TLK1 at Ser-743, preventing ASF1A phosphorylation and enhancing chromatin assembly, and also regulates PCNA for replication fork maintenance. Related references: PMID:12660173, PMID:12955071, PMID:18451105
• Additionally, Chk1 phosphorylates histone H3.1 (H3T11ph) to epigenetically silence genes, phosphorylates RB1 to promote E2F interaction, and targets SPRTN and PABIR1/FAM122A to modulate replication stress and G2/M checkpoint. Related references: PMID:17380128, PMID:31316063, PMID:33108758
• An endogenous splice variant acts as a repressor of full-length Chk1, antagonizing its function to promote S to G2/M transition.
• Subcellularly, Chk1 localizes to the nucleus, chromosomes, cytoplasm, and centrosomes, with enrichment at the centrosome during mitosis.
• Expressed ubiquitously, with highest levels in thymus, testis, small intestine, and colon.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around Ser317; ensure sample preparation includes phosphatase inhibitors to preserve endogenous phosphorylation at this site.
• For Western blotting, a specific band at ~54 kDa is expected. Validate specificity by treating cell lysates with lambda phosphatase or using non‑phospho‑specific Chk1 antibodies as controls.
• In ELISA, use immobilized peptide or cell lysates and consider blocking with phosphorylated versus non‑phosphorylated peptides to confirm phospho‑specificity.
• Optimize antibody dilution for your specific experimental system; typical starting dilutions for WB are 1:500–1:2000, but empirical determination is recommended.
• Cross‑reactivity has been confirmed for human samples; for other species, verify sequence homology around Ser317 and test experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with streamlined access to a broad portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For projects targeting DNA damage response and cell cycle regulation, we can source and supply rare‑target raw materials such as phospho‑specific antibodies to Chk1 and related checkpoint kinases. Whether you need a single reagent or a complete custom panel, CamelBio is your partner from concept to clinic.