Phospho-CGAS (S420) Rabbit Polyclonal Antibody for WB and ELISA - Q8C6L5

Core Product Specifications and Parameters

Biological Background: CGAS Function and Localization

• CGAS (cyclic GMP-AMP synthase) is a nucleotidyltransferase that catalyzes the formation of 2',3'-cGAMP from ATP and GTP, a second messenger crucial for innate immunity. Related references: PMID:23258413 PMID:23647843 PMID:23722158

• Acts as a key cytosolic DNA sensor, directly binding double-stranded DNA to induce liquid-like droplets that activate CGAS, leading to STING1-dependent type-I interferon production. Related references: PMID:23722158 PMID:28095500 PMID:28314590

• Preferentially binds long dsDNA (around 45 bp) and forms ladder-like networks that cooperatively stabilize cGAS-dsDNA complexes. Related references: PMID:28902841

• Detects foreign DNA from viruses, retroviruses, and bacteria, and also senses endogenous self-DNA leaked during cellular stress (e.g., mitochondrial stress, senescence, micronuclei formation). Related references: PMID:23258413 PMID:23647843 PMID:23722158 PMID:23929945 PMID:28738408 PMID:28759028

• 2',3'-cGAMP can transfer to neighboring cells via gap junctions or viral particles, propagating antiviral immunity. Related references: PMID:24077100 PMID:26229117 PMID:31992625

• In healthy cells, nuclear CGAS is kept inactive by chromatin binding, preventing autoimmunity; however, it also suppresses DNA repair by interacting with PARP1. Related references: PMID:30356214 PMID:31544964 PMID:31808743 PMID:32911480 PMID:32911481 PMID:35210392

• Subcellular localization includes nucleus, chromosomes, cytoplasm, cytosol, and cell membrane. The protein is subject to multiple post-translational modifications such as phosphorylation, acetylation, and palmitoylation, which regulate its activity and localization.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around serine 420 of mouse CGAS. This design ensures specificity for the phosphorylated form. Consider using phosphatase treatment on samples to confirm phospho-specificity in Western blot.
• For Western blot (WB), a band around 58kDa is expected based on the predicted molecular weight. Validate antibody dilution and exposure times in your sample system.
• For ELISA, pair with an appropriate detection antibody and optimize coating antigen and antibody concentrations. Test cross-reactivity with human and rat CGAS to confirm reactivity.
• Perform proper controls (unstimulated vs. stimulated cells, phosphatase-treated lysates) to ensure the detected signal is phosphorylation-dependent.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio accelerates your IVD development by providing comprehensive raw material solutions—from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing. This Phospho-CGAS-S420 antibody supports research into innate immunity and the cGAS-STING pathway, and CamelBio can complement your program with additional validated reagents, custom antibody development, and technical consultation to streamline your diagnostic pipeline.