Phospho-CFL1-S3 Rabbit pAb for WB, IHC-P, IF/ICC, ELISA - P23528

Core Product Specifications and Parameters

Biological Background: Cofilin-1 Function and Localization

• Cofilin-1 (CFL1) is an 18 kDa actin-binding phosphoprotein, also known as p18 or non-muscle cofilin, encoded by the CFL1 gene (GeneID: 1072). It is widely expressed across human tissues.
• The protein reversibly binds filamentous actin (F-actin) and exhibits pH-sensitive F-actin depolymerizing activity, regulating actin filament dynamics and turnover (PubMed:11812157, PubMed:33670794).
  Related references: PMID:11812157, PMID:33670794
• Cofilin-1 is essential for early embryonic development: in conjunction with the subcortical maternal complex, it regulates actin dynamics to ensure proper mitotic spindle centralization and symmetric zygotic division (By similarity).
• It plays a critical role in cell morphology and cytoskeletal organization in epithelial cells (PubMed:21834987).
  Related references: PMID:21834987
• By modulating actin, cofilin-1 facilitates the trafficking and upregulation of atypical chemokine receptor ACKR2 from endosomes to the cell membrane, enhancing chemokine clearance (PubMed:23633677).
  Related references: PMID:23633677
• Subcellularly, cofilin-1 localizes to the nucleus matrix, cytoskeleton, cell ruffles, lamellipodia, growth cones, and axons, reflecting its roles in nuclear actin dynamics and cell migration.
• The protein undergoes multiple post-translational modifications (acetylation, phosphorylation, ubiquitination) that regulate its activity; phosphorylation at the N‑terminus (e.g., Ser3) is a key switch for actin binding and is targeted by this antibody.
• Cofilin-1 is involved in neural tube morphogenesis and neural crest cell migration, underscoring its importance in developmental processes (By similarity).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide spanning Ser3 of human CFL1. For phospho-specific detection, always include appropriate controls (e.g., phosphatase-treated lysates).
• For Western blotting, use freshly prepared lysis buffer supplemented with phosphatase inhibitors and avoid excessive sonication to preserve the phosphate epitope. The predicted molecular weight is 19 kDa.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), verify signal specificity by comparing with total CFL1 staining or peptide competition.
• For ELISA applications, consider a sandwich format with a capture antibody targeting a distant epitope to confirm phospho-specificity.

CamelBio: Your One-Stop Sourcing Bridge

As your one-stop IVD raw material partner, CamelBio streamlines the sourcing of specialized reagents like Phospho-CFL1-S3 monoclonal and polyclonal antibodies, validated antibody pairs, and ancillary buffers for actin cytoskeleton research. With expertise across the entire diagnostic development pipeline, we supply bulk-quantity phospho-specific antibodies, recombinant antigens, and rare-target raw materials to support your assay manufacturing and clinical translation needs.