Phospho-C/EBPB-T235 Rabbit Polyclonal Antibody for WB, ELISA - P17676

Core Product Specifications and Parameters

Biological Background: C/EBPB Function and Localization

• C/EBPB (CCAAT/enhancer-binding protein beta) is a transcription factor that critically regulates genes involved in immune and inflammatory responses, binding to regulatory regions of acute-phase and cytokine genes. It is essential for gene expression in activated macrophages and plays a major role in CD4+ T-cell responses. Related references: PMID:12048245, PMID:1741402, PMID:18647749

• During adipogenesis, C/EBPB is rapidly expressed and, after activation by phosphorylation, induces CEBPA and PPARG, which are necessary for terminal differentiation. It allows mitotic clonal expansion essential for adipocyte maturation. Related references: PMID:20829347

• C/EBPB can act as a dominant negative through heterodimerization with isoform 2 (LIP). It also promotes osteoblast differentiation and osteoclastogenesis, highlighting its diverse roles in tissue development. Related references: PMID:11741938

• The protein localizes to both the nucleus and cytoplasm, with its functional capacity governed by protein interactions and post-translational modifications.

• Post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination, tightly regulate C/EBPB activity. Phosphorylation at T235 is one key modification, and this antibody specifically recognizes that phosphorylated state.

• C/EBPB is expressed at low levels in the lung, kidney, and spleen, suggesting tissue-specific regulatory roles.

• In addition to its transcriptional roles, C/EBPB is involved in intracellular bacteria killing (By similarity).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around T235; therefore, this antibody is specific for the phosphorylated form. Consider using phosphatase inhibitors during sample preparation to preserve the phosphorylation signal.
• The antibody is suitable for western blot (WB) and ELISA. For WB, the predicted molecular weight is approximately 36 kDa; validate the band in your sample system with appropriate controls.
• Cross-reactivity is reported for human and mouse; validate reactivity in other species if required.
• As C/EBPB is a transcription factor, nuclear or whole-cell extracts may be explored to improve detection depending on the experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop IVD raw-material sourcing partner, offering a comprehensive range of validated antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This product targets C/EBPB, a transcription factor central to immune response and adipogenesis, and can be integrated into research or diagnostic workflows. Leverage CamelBio's supply capabilities to access optimized monoclonal/polyclonal antibodies and complementary raw materials for your assay development, from concept to clinic.