Phospho-CDKN1B/p27KIP1-T187 Rabbit pAb for WB - P46527

Core Product Specifications and Parameters

Biological Background: CDKN1B/p27KIP1 Function and Localization

• CDKN1B encodes the cyclin-dependent kinase inhibitor 1B, also known as p27Kip1, a critical regulator of cell cycle progression.
• It inhibits the kinase activity of CDK2 bound to cyclin A, while showing little effect on CDK2-SPDYA complexes, and potently blocks cyclin E- and cyclin A-CDK2 activity, promoting G1 arrest. Related references: PMID:28666995
• p27Kip1 forms complexes with cyclin D-CDK4 and modulates CCND1-CDK4 complex assembly and activation, acting as either an inhibitor or activator depending on phosphorylation state.
• Subcellularly, p27 resides primarily in the nucleus but can also localize to the cytoplasm and endosomes, with its localization influencing its regulatory functions.
• Tissue expression is broad, with protein detected in all tissues tested; highest expression is observed in skeletal muscle, while lower levels are found in liver and kidney. Related references: PMID:15509543, PMID:8033212
• The predicted molecular weight is approximately 22 kDa.
• p27 is classified as a phosphoprotein with protein kinase inhibitor and tumor suppressor activities, and it undergoes ubiquitin conjugation, reflecting its tight post-translational regulation.

Experimental Guidance and Technical Tips

• Since this antibody is raised against a phospho-specific peptide surrounding T187, it likely detects CDKN1B/p27KIP1 specifically when phosphorylated at this residue. Validate phosphorylation specificity using phosphatase-treated samples or non-phosphorylatable mutants.
• For Western blot (WB), consider using fresh cell or tissue lysates prepared with phosphatase inhibitors to preserve the phosphorylation signal; a band near 22 kDa is expected.
• Given the ubiquitous tissue expression, multiple human cell lines or tissue types can serve as positive controls; however, signal intensity may vary with cell cycle status.
• The antibody's rabbit polyclonal nature may provide robust detection across various denaturing and reducing conditions; optimize blocking and dilution conditions experimentally.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio provides researchers and manufacturers with integrated access to antibodies like this phospho-p27KIP1 polyclonal antibody, supporting cell cycle and oncology research. We offer customizable sourcing options, including validated antibody pairs, bulk ancillary reagents, and streamlined procurement for rare or phosphorylated targets. Contact our team to discuss your specific assay development needs.