Phospho-CDK6-Y24 Rabbit pAb for WB/IF/ICC - Q00534

Core Product Specifications and Parameters

Biological Background: CDK6 Function and Localization

• Cyclin-dependent kinase 6 (CDK6), also known as cell division protein kinase 6 or serine/threonine-protein kinase PLSTIRE, is a member of the cyclin-dependent kinase family that controls cell cycle progression and differentiation.
• CDK6 promotes G1/S transition by phosphorylating pRB/RB1 and NPM1; it interacts with D-type cyclins to form active kinases that regulate cell cycle entry.
• Involved in both proliferation of specific cell types (e.g., erythroid and hematopoietic cells) and maintenance of cell cycle exit during differentiation; also required for thymocyte development.
• Essential for cell proliferation in the hippocampal dentate gyrus and subventricular zone; promotes newborn neuron production likely by modulating G1 length.
• In astrocytes, CDK6 induces gene expression changes, actin cytoskeleton reorganization, and enhanced motility; prevents myeloid differentiation by interfering with RUNX1 while supporting normal myeloid progenitor proliferation.
• Delays cellular senescence and promotes beta-cell proliferation in pancreatic islets of Langerhans.
• May play a role in centrosome organization during the cell cycle phases. Related references: PMID:23918663
• Subcellular localization includes cytoplasm, nucleus, cell projections (ruffles), and centrosome; broadly expressed, with accumulation in certain cancers and proliferating progenitor cells.

Experimental Guidance and Technical Tips

• Due to the phospho‑specific nature of the immunogen, confirm phospho‑specificity via phosphatase treatment or peptide competition assays using non‑phosphorylated peptide.
• For Western blot (WB), include positive control lysates from proliferating human or mouse cell lines known to express phosphorylated CDK6 (e.g., cancer lines).
• In immunofluorescence/immunocytochemistry (IF/ICC), validate staining with appropriate stimuli or inhibitors that modulate CDK6 phosphorylation at Tyr24.
• The reported cross‑reactivity with human and mouse suggests utility in both species; verify reactivity in the specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing diagnostic manufacturers and laboratories with validated antibodies, bulk ancillary reagents, and customized procurement solutions. This phospho‑CDK6 antibody supports cell cycle, oncology, and differentiation research, and CamelBio streamlines access to optimized monoclonal/polyclonal antibodies and rare‑target raw materials—helping you move from concept to clinic efficiently.