Phospho-CDK2-Y15 Rabbit mAb for WB, ELISA - P24941

Core Product Specifications and Parameters

Biological Background: CDK2 Function and Localization

• Cyclin-dependent kinase 2 (CDK2), also known as p33 protein kinase, is a serine/threonine-protein kinase essential for cell cycle control; it is dispensable for mitosis but required for meiosis. Related references: PMID:10499802 PMID:10884347
• Acts at the G1-S transition to promote the E2F transcriptional program and initiation of DNA synthesis, and modulates G2 progression; it activates cyclin B/CDK1 to control entry into mitosis. Related references: PMID:18372919 PMID:19238148 PMID:19561645
• Phosphorylates multiple substrates including RB1, BRCA2, MYC, and EZH2, regulating DNA replication, DNA repair, transcription, and epigenetic silencing. Related references: PMID:10499802 PMID:19966300 PMID:20935635
• Triggers duplication of centrosomes and DNA by phosphorylating NPM1, causing its dissociation from centrosomes and initiating duplication. Related references: PMID:11051553
• Found in the nucleus, Cajal body, centrosome, cytoplasm, and endosome, reflecting its diverse regulatory roles.
• Involved in the G1-S DNA damage checkpoint; phosphorylates BRCA2 and NBN to regulate homologous recombination-dependent repair and telomere maintenance. Related references: PMID:15800615 PMID:21319273 PMID:28216226
• Post-translational modifications including acetylation, phosphorylation at Tyr15 (inhibitory), Ser/Thr sites, and S-nitrosylation fine-tune its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around Tyr15; use phosphatase inhibitors during sample preparation to preserve phosphorylation.
• For Western blot, expect a ~34 kDa band; validate in your cell/tissue system with positive controls treated with phosphatase inhibitors or cell cycle-phase specific activators.
• ELISA application allows detection of phospho-CDK2 levels; optimize coating and detection conditions for your assay format.
• The antibody cross-reacts with human and mouse CDK2; for other species, check sequence homology around Tyr15 before use.

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