Phospho-Cdk1/2-T14 Rabbit Monoclonal Antibody for WB, ELISA - P06493 / P24941

Core Product Specifications and Parameters

Biological Background: CDK1/CDK2 Function and Localization

• CDK1 (Cyclin-dependent kinase 1), also known as CDC2 or p34 protein kinase, is a master regulator of the eukaryotic cell cycle, promoting the G2-M transition through association with multiple interphase cyclins and phosphorylation of numerous substrates. Related references: PMID:16407259 PMID:16933150 PMID:17459720

• Phosphorylation at Thr-14 (and Tyr-15) by WEE1 and PKMYT1 kinases inhibits CDK1 activity, providing a crucial checkpoint for DNA damage response and proper mitotic timing. Related references: PMID:18480403 PMID:20360007

• Dephosphorylation at Thr-14 by CDC25 phosphatases activates CDK1-cyclin complexes, triggering chromosome condensation, nuclear envelope breakdown, and spindle assembly. Related references: PMID:18480403 PMID:20360007 PMID:2188730

• CDK1 localizes to the nucleus, cytoplasm, mitochondria, centrosome, and mitotic spindle, reflecting its diverse roles during cell division.

• CDK1 is expressed in proliferating tissues and is found in breast cancer, where its dysregulation contributes to uncontrolled cell proliferation.

• Beyond mitosis, CDK1 participates in apoptosis, circadian rhythm regulation, and serves as a host cell receptor for hepatitis C virus entry.

• The CDK2 paralog shares high homology and is similarly regulated by Thr-14 phosphorylation, extending the antibody's utility in cell cycle research.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around Thr-14 of human CDK1, with predicted cross-reactivity to CDK2; consider verifying specificity via phosphatase treatment or competitive inhibition with non-phosphorylated peptide.

• For western blotting, the observed band is expected near 34 kDa; signal intensity may vary with cell cycle phase, so using synchronized cell populations or mitotic markers is advisable.

• In ELISA applications, pre-coating with the phosphopeptide antigen and proper blocking can enhance assay sensitivity; always include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials and technical services, from concept to clinic. This Phospho-Cdk1/2-T14 Rabbit mAb addresses critical needs in cell cycle and oncology research, backed by our reliable supply chain and expertise in antibody development. Whether you need validated antibody pairs, bulk monoclonal antibodies, or rare target raw-material sourcing, we are your trusted partner for accelerating assay development.