Phospho-CDK1-Y15 Rabbit mAb for WB, ELISA - P06493

Core Product Specifications and Parameters

Biological Background: CDK1 Function and Localization

• Cyclin-dependent kinase 1 (CDK1), also known as CDC2 or p34 protein kinase, is a serine/threonine-protein kinase that plays a pivotal role in the eukaryotic cell cycle, promoting the G2-M transition and mitotic onset (PubMed:16407259, PubMed:16933150, PubMed:17459720). Related references: PMID:16407259 PMID:16933150 PMID:17459720
• It phosphorylates a wide array of substrates—including lamins, histone H1, tau, and vimentin—to orchestrate centrosome separation, nuclear envelope breakdown, and chromosome condensation (PubMed:16407259, PubMed:16933150, PubMed:17459720, PubMed:18480403, PubMed:20360007, PubMed:2188730). Related references: PMID:16407259 PMID:16933150 PMID:17459720 PMID:18480403 PMID:20360007 PMID:2188730
• CDK1 activity is tightly regulated by phosphorylation/dephosphorylation at Thr14 and Tyr15. WEE1- and PKMYT1-mediated phosphorylation at Tyr15 inhibits CDK1, while CDC25 phosphatases remove this inhibitory phosphate to drive mitotic entry (PubMed:18480403, PubMed:20360007, PubMed:2188730, PubMed:20395957). Related references: PMID:18480403 PMID:20360007 PMID:2188730 PMID:20395957
• In response to DNA damage, CDK1 is phosphorylated at Tyr15 and inhibited, leading to G2 arrest for DNA repair; the phosphatase WIP1 reactivates CDK1 after repair is complete (PubMed:20360007, PubMed:20395957). Related references: PMID:20360007 PMID:20395957
• CDK1 localizes to the nucleus, cytoplasm, mitochondria, centrosome, and spindle, reflecting its multiple mitotic roles.
• Found at elevated levels in breast cancer tissues, making it a potential diagnostic and therapeutic target.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide encompassing Tyr15 of human CDK1; therefore, the antibody specifically recognizes the phosphorylated form.
• For Western blotting, expect a band at ~34 kDa. Use phosphatase inhibitors in lysis buffer to preserve phosphorylation.
• Specificity can be verified by treating samples with lambda phosphatase or by performing peptide competition assays with the phosphorylated and non-phosphorylated peptide.
• This antibody is validated for human samples; cross-reactivity with other species has not been determined.
• For ELISA, consider using the phosphopeptide as capture antigen and a total CDK1 antibody as detection for normalization.

CamelBio: Your One-Stop Sourcing Bridge

As your one-stop sourcing partner for IVD raw materials, CamelBio offers a comprehensive range of validated antibodies, including phospho-specific CDK1 antibodies, to support cell cycle and oncology research. We supply antibody pairs, monoclonal and polyclonal reagents, bulk ancillary materials, and can source rare targets to meet your diagnostic development needs. Contact us to discover how we can accelerate your project from concept to clinic.