Phospho-CDK1-T161 Rabbit pAb - P06493

Core Product Specifications and Parameters

Biological Background: CDK1 Function and Localization

• CDK1 (also known as CDC2) is a serine/threonine-protein kinase essential for G2-M transition in the cell cycle, phosphorylating numerous substrates to drive mitotic events. Related references: PMID:16407259, PMID:16933150, PMID:17459720
• Its activation requires phosphorylation at Thr161 by the CDK-activating kinase (CAK) complex, while inhibitory phosphorylation at Thr14 and Tyr15 by WEE1 and PKMYT1 maintains it inactive; dephosphorylation by CDC25 phosphatases triggers mitotic entry. Related references: PMID:18480403, PMID:20360007, PMID:2188730, PMID:41100585
• CDK1 phosphorylates nuclear lamins (LMNA, LMNB1, LMNB2) causing nuclear envelope breakdown during mitosis. Related references: PMID:2188730, PMID:2344612, PMID:37788673
• Upon DNA damage, CDK1 is inactivated via PKR/WEE1 to enforce G2 arrest, and reactivated after repair through WIP1/CDC25 signaling. Related references: PMID:20360007, PMID:20395957
• CDK1 localizes to multiple subcellular compartments including the nucleus, cytoplasm, mitochondrion, centrosome, and mitotic spindle, enabling coordination of diverse mitotic processes.
• CDK1 participates in circadian clock regulation by phosphorylating NR1D1, marking it for degradation; it also acts as a receptor for hepatitis C virus entry into hepatocytes. Related references: PMID:27238018

Experimental Guidance and Technical Tips

• Since this antibody targets a phosphorylation site (Thr161), maintain phospho-status during sample preparation by including phosphatase inhibitors and avoiding heating in phosphate-free buffers.
• For Western blot, a band at approximately 34 kDa is expected; consider using mitotic cell lysates (e.g., cells arrested at G2/M) as positive controls and lambda phosphatase treatment as a negative control.
• In immunoprecipitation, the antibody's performance may depend on the abundance of the phospho-epitope; enrich for mitotic cells to enhance detection.
• For immunofluorescence and IHC, standard antigen retrieval coupled with rapid fixation can preserve the phosphorylation signal; pilot experiments to optimize detection are advised.
• Although listed for ELISA, direct antigen coating with the phosphopeptide may be required; competitive ELISA can be designed to quantify phosphorylation levels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this phospho-CDK1-T161 antibody as part of a comprehensive portfolio for cell cycle and cancer research. With a one-stop supply model, we provide validated antibody pairs, optimized recombinant antibodies, and ancillary reagents, ensuring seamless integration into IVD assay development. Our team assists in sourcing rare phosphorylation-specific reagents to accelerate your diagnostic projects.