Phospho-Cdc6-S54 Rabbit mAb for WB, ELISA - Q99741

Core Product Specifications and Parameters

Biological Background: CDC6 Function and Localization

• CDC6 (Cell division control protein 6 homolog) is a critical initiator of DNA replication, responsible for loading the minichromosome maintenance (MCM) helicase complex onto replication origins.
• It also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated, maintaining genomic stability.
• The protein shuttles between the nucleus and cytoplasm; its subcellular localization is tightly regulated during the cell cycle.
• Phosphorylation at Serine 54 (S54) is a key post-translational modification that modulates CDC6 activity, stability, and nuclear export, linking it to cell cycle progression.
• CDC6 is classified as a phosphoprotein, ATP-binding protein, and ubiquitin-conjugated protein (Ubl conjugation), reflecting its dynamic regulation.
• Mutations in the CDC6 gene are associated with Meier-Gorlin syndrome type 5 (MGORS5), a disorder characterized by dwarfism, microtia, and patellar aplasia/hypoplasia.

Experimental Guidance and Technical Tips

• For Western blotting, use a positive control cell line known to express CDC6 (e.g., HeLa or HEK293) and expect a band at approximately 63 kDa.
• To confirm phosphorylation specificity at S54, consider treating samples with lambda phosphatase or kinase/phosphatase inhibitors prior to analysis.
• Cross-reactivity with mouse CDC6 has been reported; validate performance in your specific mouse tissue or cell line.
• In ELISA applications, use the phosphorylated S54 peptide as a coating antigen and include appropriate negative controls (e.g., non-phosphorylated peptide).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies diagnostic manufacturers, contract research organizations, and laboratories with validated IVD raw materials for every stage from concept to clinic. Our portfolio includes anti-phospho-CDC6 (S54) antibodies for DNA replication and cell cycle research, along with complementary antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing. Partner with us to streamline your assay development and secure consistent, performance-grade reagents.