Phospho-CBL-Y700 Rabbit Polyclonal Antibody for WB and ELISA - P22681

Core Product Specifications and Parameters

Biological Background: CBL Function and Localization

• CBL is an E3 ubiquitin-protein ligase that negatively regulates many signaling pathways by mediating ubiquitination of cell surface receptors, targeting them for proteasomal degradation (PubMed:10514377, PubMed:11896602, PubMed:14661060). Related references: PMID:10514377, PMID:11896602, PMID:14661060

• It recognizes activated receptor tyrosine kinases including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, CSF1R, EPHA8 and KDR, and mediates their ubiquitination to terminate signaling (PubMed:15190072, PubMed:18374639, PubMed:21596750). Related references: PMID:15190072, PMID:18374639, PMID:21596750

• CBL also ubiquitinates non-receptor tyrosine kinases such as HCK and SRC family members, promoting their degradation (PubMed:11896602). Related references: PMID:11896602

• Ubiquitinates EGFR and SPRY2, influencing downstream signaling (PubMed:17094949, PubMed:17974561). Related references: PMID:17094949, PMID:17974561

• Involved in LAG3-mediated inhibition of TCR signaling by catalyzing Lys-63-linked ubiquitination of LAG3 (PubMed:40101708). Related references: PMID:40101708

• Mediates ubiquitination of NECTIN1 upon herpes simplex virus 1 infection, leading to NECTIN1 removal from the cell surface (PubMed:28381567). Related references: PMID:28381567

• Plays a critical role in osteoclast function; the Tyr-731 phosphorylated form recruits PI3K, essential for bone resorption (PubMed:14739300). Related references: PMID:14739300

• Localizes to the cytoplasm, cell membrane, cell projections (cilium), and Golgi apparatus.

• Contains a RING finger domain, is a metal-binding phosphoprotein, and functions as a proto-oncogene.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Tyr700 of human CBL. Verify antibody specificity using non-phosphorylated peptide competition or phosphatase treatment.
• For western blotting, the expected band is ~100 kDa. Include appropriate molecular weight markers and validate with positive and negative controls.
• This antibody is listed for WB and ELISA. Optimize antibody dilution and incubation conditions for your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw material supplier, CamelBio offers comprehensive access to validated antibodies, bulk reagents, and technical support. For ubiquitination and tyrosine kinase signaling research, this phospho-CBL antibody can be integrated into your assay development workflow. Contact us for one-stop sourcing spanning from concept to clinic.