Phospho-c-Myc-T58/S62 Rabbit mAb for WB, ELISA - P01106

Core Product Specifications and Parameters

Biological Background: c-Myc Function and Localization

• Myc proto-oncogene protein (c-Myc) is a transcription factor that binds DNA non-specifically and specifically recognizes the E-box sequence 5'-CAC[GA]TG-3', activating growth-related genes. Related references: PMID:24940000, PMID:25956029
• It binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis. Related references: PMID:24940000, PMID:25956029
• c-Myc regulates somatic reprogramming and controls self-renewal of embryonic stem cells.
• It cooperates with TAF6L to activate gene expression by releasing RNA polymerase II pausing.
• c-Myc positively regulates transcription of HNRNPA1, HNRNPA2, and PTBP1, which modulate alternative splicing of PKM, favoring the PKM2 isoform. Related references: PMID:20010808
• Subcellularly, c-Myc localizes to the nucleus (nucleoplasm, nucleolus) and cytoplasm, and associates with chromosomes.
• It undergoes various post-translational modifications, including phosphorylation, acetylation, O-glycosylation, and ubiquitination, which govern its activity and stability.

Experimental Guidance and Technical Tips

• When performing western blot, use phosphatase inhibitor cocktails during cell lysis to preserve phosphorylation signals at T58 and S62.
• For ELISA, consider using phosphorylated peptide as a positive control to confirm antibody specificity.
• Validate the antibody's phospho-specificity by comparing signals with a pan-c-Myc antibody or after phosphatase treatment.
• Since the antibody is a rabbit monoclonal, it provides high specificity and lot-to-lot consistency for quantitative assays.
• Cross-reactivity with rat c-Myc has been noted; optimize conditions for rat samples accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio facilitates your IVD raw-material sourcing with a comprehensive portfolio of antibodies, including this phospho-specific rabbit monoclonal antibody targeting c-Myc, a pivotal oncogene in cancer research and diagnostics. Beyond this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, ensuring seamless integration into your assay development pipelines.