Phospho-c-Myc-S62 Rabbit Monoclonal Antibody for WB and ELISA - P01106

Core Product Specifications and Parameters

Biological Background: c-Myc Function and Localization

• Myc proto-oncogene protein (c-Myc), also known as transcription factor p64 or class E basic helix-loop-helix protein 39 (bHLHe39), is a member of the MYC family of transcription factors.
• Functions as a transcription factor that binds the core DNA sequence 5'-CAC[GA]TG-3' and activates the transcription of growth-related genes, including VEGFA, to promote angiogenesis.
  Related references: PMID:24940000 PMID:25956029
• Regulates somatic reprogramming and controls self-renewal of embryonic stem cells.
• Positively regulates alternative splicing of pyruvate kinase PKM by activating HNRNPA1, HNRNPA2, and PTBP1 transcription, thereby inhibiting exon 9 inclusion and promoting the PKM M2 isoform.
  Related references: PMID:20010808
• Localizes primarily to the nucleus, including the nucleoplasm and nucleolus, but can also be found in the cytoplasm and associated with chromosomes.
• Subject to multiple post-translational modifications, including phosphorylation at residues such as S62, acetylation, glycosylation, and ubiquitination, which regulate protein stability and transcriptional activity.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic phosphorylated peptide around S62 of human c-Myc, designed for phospho-specificity. For Western blotting, consider using phosphatase inhibitors to preserve phosphorylation status in cell or tissue lysates.
• Validate the antibody in your sample system with appropriate controls, such as phosphatase-treated samples or non-phosphorylated peptide competition, to confirm phospho-specific detection.
• For ELISA applications, test antibody binding to phosphorylated versus non-phosphorylated peptide antigens to assess specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target material sourcing. This phospho-c-Myc (S62) antibody targets a critical oncogenic transcription factor, supporting cancer research and diagnostic assay development. From concept to clinic, our team provides the raw materials and technical services you need to accelerate your project.