Phospho-c-Jun-T93 Rabbit pAb for WB, ELISA - P05412

Core Product Specifications and Parameters

Biological Background: c-Jun Function and Localization

• Recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3' to regulate transcription. Related references: PMID:10995748, PMID:22083952

• Heterodimerizes with FOS family proteins to form the activator protein-1 (AP-1) transcription complex.

• Together with FOSB, c-Jun regulates activation-induced T cell death by binding to the AP-1 site in the FASLG/CD95L promoter and inducing expression. Related references: PMID:12618758

• When phosphorylated by HIPK3, c-Jun promotes NR5A1 activity, leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Related references: PMID:17210646

• Mediates activated KRAS-driven transcriptional activation of USP28 in colorectal cancer cells by binding to the USP28 promoter. Related references: PMID:24623306

• Upon Epstein-Barr virus (EBV) infection, c-Jun transactivates viral BZLF1 expression by binding to the BZLF1 Z promoter.

• Primarily localizes to the nucleus; expressed in developing and adult prostate and prostate cancer cells; classified as a phosphoprotein, with phosphorylation at Thr93 being a key regulatory modification.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Thr93 of human c-Jun; thus, this antibody specifically recognizes the phosphorylated form. Verify phosphorylation-dependent binding by treating samples with phosphatases.
• For Western blot, the expected molecular weight is approximately 36 kDa. Include appropriate positive controls (e.g., stimulated cells) and phospho-specific inhibition controls.
• In ELISA, consider pairing with a total c-Jun antibody for normalization. Optimize coating and detection conditions to distinguish phospho-specific signal.
• Cross-reactivity with mouse c-Jun allows application in mouse model studies; validate the antibody's performance in the relevant species and tissue.
• As with any phospho-specific antibody, confirm specificity in your specific experimental context, as c-Jun phosphorylation at Thr93 may vary with cell type and stimulation conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, supporting research and diagnostic development from concept to clinic. For studies on the AP-1 transcription factor complex and c-Jun signaling, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies (including phospho-specific reagents), and bulk ancillary materials. Our global supply network also enables sourcing of rare or custom targets to accelerate your assay and product development timelines.