Phospho-c-Jun-T91 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P05412

Core Product Specifications and Parameters

Biological Background: Transcription factor Jun Function and Localization

• Transcription factor Jun (c-Jun, AP1, p39) is a core component of the AP-1 transcription factor complex, binding the consensus motif 5'-TGA[GC]TCA-3' to regulate gene expression. Related references: PMID:10995748 PMID:22083952
• Heterodimerizes with proteins of the FOS family to enhance DNA binding and transcriptional activity at AP-1 sites.
• Together with FOSB, mediates activation-induced cell death of T cells by inducing FASLG/CD95L expression following TCR/CD3 signaling. Related references: PMID:12618758
• When phosphorylated by HIPK3, enhances NR5A1 activity to increase steroidogenic gene expression upon cAMP signaling. Related references: PMID:17210646
• Participates in oncogenic KRAS-mediated transcriptional activation of USP28 in colorectal cancer cells by binding its promoter. Related references: PMID:24623306
• Localized predominantly in the nucleus.
• Expressed in developing and adult prostate, as well as prostate cancer cells.
• Regulated by phosphorylation; phosphorylation at Thr91 by JNK kinases enhances transcriptional activity and is a key event in stress-activated signaling pathways.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Thr91 of human JUN, ensuring specificity for the phosphorylated form. Verify reactivity using both phosphorylated and non-phosphorylated control samples in your assay system.
• For Western blot, expect a band at approximately 36 kDa; use phosphatase inhibitors in lysis buffers to preserve phosphorylation.
• In IHC-P and IF/ICC, appropriate fixation and antigen retrieval should be optimized; confirm staining patterns with known positive and negative tissue or cell samples.
• ELISA applications may utilize this antibody as a capture or detection reagent for phospho-c-Jun quantification; validate with recombinant proteins or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this phospho-specific anti-c-Jun antibody as part of a comprehensive portfolio of phosphorylated antibodies and transcription factor reagents for diagnostic and research applications. We supply validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents to support your assay development from concept to clinic. For customized sourcing of rare targets or technical consultation, contact CamelBio to streamline your IVD raw-material procurement.