Phospho-c-Jun-S73 Rabbit pAb - P05412

Core Product Specifications and Parameters

Biological Background: c-Jun Function and Localization

• c-Jun is a transcription factor and core component of the AP-1 complex, binding to the consensus DNA sequence 5'-TGA[GC]TCA-3' to regulate gene expression. Related references: PMID:10995748, PMID:22083952
• It heterodimerizes with members of the FOS family, enhancing DNA binding and transcriptional activation at AP-1 sites.
• In T cells, c-Jun, together with FOSB, promotes activation-induced cell death by upregulating FASLG transcription. Related references: PMID:12618758
• Phosphorylation by HIPK3 enhances c-Jun's co-activation of NR5A1, increasing steroidogenic gene expression. Related references: PMID:17210646
• In colorectal cancer, c-Jun mediates KRAS-driven transcriptional activation of USP28. Related references: PMID:24623306
• c-Jun is localized to the nucleus and is expressed in the developing and adult prostate, as well as in prostate cancer cells.
• It is subject to multiple post-translational modifications, including phosphorylation, acetylation, and ubiquitination, which modulate its activity.
• During Epstein-Barr virus infection, c-Jun binds to the viral BZLF1 promoter to activate viral gene expression.

Experimental Guidance and Technical Tips

• The antibody was generated against a synthetic phospho-peptide encompassing Ser73; validate phospho-specificity in your system using phosphatase treatment or a non-phospho peptide competition assay.
• For Western blot, expect a band around 36 kDa; include positive controls (e.g., stimulated cells) and consider using a total c-Jun antibody to confirm phosphorylation status.
• In IHC-P, optimize antigen retrieval conditions and use known positive tissues (e.g., prostate cancer) as controls; nuclear staining is anticipated.
• For ELISA, pair this antibody with a suitable capture or detection antibody to quantify phospho-c-Jun (S73) levels; validate matrix effects and dynamic range.

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